New analytical method for the study of metabolism of swertiamarin in rats after oral administration by UPLC-TOF-MS following DNPH derivatization.
New analytical method for the study of metabolism of swertiamarin in rats after oral administration by UPLC-TOF-MS following DNPH derivatization.
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DNPH衍生化后采用UPLC-TOF-MS研究大鼠口服獐牙菜苦苷代谢的新分析方法。
DOI：
10.1002/bmc.3406
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发表时间：
2015-08
期刊：
影响因子：
--
通讯作者：
Wang Zhigang
中科院分区：
文献类型：
--
作者：
Wu Xihong;Tang Shuhan;Jin Yan;Zhang Ya;Hattori Masao;Wang Xijun;Wang Zhigang
The metabolism of swertiamarin in vivo was studied by LC-MS following 2,4-dinitrophenylhydrazine derivatization. The ionization efficiency of the main metabolite erythrocentaurin was greatly enhanced by the new analytical method developed, and erythrocentaurin was successfully detected for the first time in rat plasma after oral administration of swertiamarin. Methyl 4-formylbenzoate was used as the internal standard to quantify erythrocentaurin in rat plasma in negative mode by UPLC-TOF-MS, and it was found that erythrocentaurin reached the maximum mean plasma concentration of 425.8 ± 127.6 ng/mL at about 2 h after oral administration of swertiamarin at a dose of 200 mg/kg. A metabolic pathway of swertiamarin to erythrocentaurin was proposed. Swertiamarin is first hydrolyzed by bacterial β-glucusidase to give the aglycone, which is readily converted to erythrocentaurin. The monoterpene compound swertiamarin was found to be metabolized to dihydroisocoumarin and alkaloid compounds in vivo, which may be responsible for the pharmacological effect of swertiamarin. The results may shed light on the clinical efficacy of swertiamarin and the new analytical method may assist in studies for the metabolism of other natural iridoids and secoiridoids in vivo.
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影响因子：
1.7
作者：
Zhigang Wang;Xi-jun Wang;Hui Sun;Li Chen;Chao-Mei Ma
通讯作者：
Zhigang Wang;Xi-jun Wang;Hui Sun;Li Chen;Chao-Mei Ma
影响因子：
5.4
作者：
J. Yamahara;M. Kobayashi;H. Matsuda;S. Aoki
通讯作者：
J. Yamahara;M. Kobayashi;H. Matsuda;S. Aoki
DOI：
10.1016/j.jchromb.2006.10.036
发表时间：
2007-04-01
影响因子：
3
作者：
Higashi, Tatsuya;Nagahama, Akihiro;Shimada, Kazutake
通讯作者：
Shimada, Kazutake
影响因子：
7.9
作者：
Kumarasamy, Y;Nahar, L;Sarker, SD
通讯作者：
Sarker, SD
影响因子：
2.7
作者：
A. I. el-Sedawy;Y. Shu;M. Hattori;K. Kobashi;T. Namba
通讯作者：
A. I. el-Sedawy;Y. Shu;M. Hattori;K. Kobashi;T. Namba