The Use of Collagen Methacrylate in Actuating Polyethylene Glycol Diacrylate–Acrylic Acid Scaffolds for Muscle Regeneration
The Use of Collagen Methacrylate in Actuating Polyethylene Glycol Diacrylate–Acrylic Acid Scaffolds for Muscle Regeneration
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DOI：
10.1007/s10439-023-03139-8
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发表时间：
2023-02
影响因子：
3.8
通讯作者：
Yoliem S Miranda Alarcón;D. Jazwinska;Terrence Lymon;A. Khalili;D. Browe;Brandon Newton;M. Pellegrini;Rick I Cohen;D. Shreiber;J. Freeman
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文献类型：
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作者：
Yoliem S Miranda Alarcón;D. Jazwinska;Terrence Lymon;A. Khalili;D. Browe;Brandon Newton;M. Pellegrini;Rick I Cohen;D. Shreiber;J. Freeman
在肌肉损失或损伤后，骨骼肌组织具有再生和恢复其功能的能力。然而，骨骼肌组织中的大体积缺陷由于缺乏诸如生物物理和生物化学线索的再生元素而对再生提出了挑战，使得有必要开发新的治疗方法。一种潜在的解决方案是利用可以响应于外部电场而改变尺寸或形状的电活性聚合物。聚（乙二醇）二丙烯酸酯（PEGDA）就是这样一种聚合物，由于其机械性能，其作为肌肉组织再生支架具有很大的潜力。此外，这种聚合物的多功能化学允许新官能团的共轭，以增强其电活性和生物相容性。本文中，我们开发了PEGDA和丙烯酸（AA）的电活性共聚物与胶原甲基丙烯酸酯（CMA）组合以促进细胞粘附和增殖。CMA + PEGDA：AA构建体的电活性性质通过致动研究进行了研究。此外，在14天的体外研究中研究了水凝胶的生物学特性，以评估肌球蛋白轻链（MLC）的表达和C2C12小鼠成肌细胞的代谢活性。CMA的加入改善了材料生物活性的某些方面，例如MLC在C2C12小鼠成肌细胞中的表达。然而，CMA的PEGDA：AA水凝胶中的掺入减少了样品移动时，放置在电场下，可能是由于空间位阻从CMA。CMA与PEGDA：AA联合应用作为骨骼肌组织工程支架的可能性有待进一步研究。
After muscle loss or injury, skeletal muscle tissue has the ability to regenerate and return its function. However, large volume defects in skeletal muscle tissue pose a challenge to regenerate due to the absence of regenerative elements such as biophysical and biochemical cues, making the development of new treatments necessary. One potential solution is to utilize electroactive polymers that can change size or shape in response to an external electric field. Poly(ethylene glycol) diacrylate (PEGDA) is one such polymer, which holds great potential as a scaffold for muscle tissue regeneration due to its mechanical properties. In addition, the versatile chemistry of this polymer allows for the conjugation of new functional groups to enhance its electroactive properties and biocompatibility. Herein, we have developed an electroactive copolymer of PEGDA and acrylic acid (AA) in combination with collagen methacrylate (CMA) to promote cell adhesion and proliferation. The electroactive properties of the CMA + PEGDA:AA constructs were investigated through actuation studies. Furthermore, the biological properties of the hydrogel were investigated in a 14-dayin vitrostudy to evaluate myosin light chain (MLC) expression and metabolic activity of C2C12 mouse myoblast cells. The addition of CMA improved some aspects of material bioactivity, such as MLC expression in C2C12 mouse myoblast cells. However, the incorporation of CMA in the PEGDA:AA hydrogels reduced the sample movement when placed under an electric field, possibly due to steric hindrance from the CMA. Further research is needed to optimize the use of CMA in combination with PEGDA:AA as a potential scaffold for skeletal muscle tissue engineering.