CYSTM3 negatively regulates salt stress tolerance in Arabidopsis
CYSTM3 negatively regulates salt stress tolerance in Arabidopsis
复制标题
CYSTM3负向调节拟南芥耐盐胁迫
DOI：
10.1007/s11103-019-00825-x
复制
发表时间：
2019-03-01
影响因子：
5.1
通讯作者：
Huang, Jinguang
中科院分区：
文献类型：
--
作者：
Xu, Yang;Yu, Zipeng;Huang, Jinguang
CYSTM 3是一种线粒体小分子蛋白，在拟南芥盐胁迫反应中起负调节作用，其作用是阻止Na+外流和干扰活性氧（ROS）的稳态。富含半胱氨酸的跨膜组件（CYSTM）是植物中一个特征不明确的小肽家族。在这项研究中，我们确定了一个新的定位于拟南芥CYSTM成员CYSTM 3，这是普遍存在于不同的组织和显着的盐胁迫诱导。与野生型（WT）植物相比，过表达CYSTM3（OE）的转基因植物表现出对盐胁迫的敏感性，而敲除突变体cystm3比WT更耐受高盐度。另外，在高盐胁迫下，OE系积累的Na+和ROS含量高于WT和cystm3。进一步的分析表明，CYSTM 3可以阻止拟南芥根系Na+外流，并抑制一系列ROS清除酶的活性。此外，cystm3细胞核盐胁迫响应基因的转录本比WT和OE细胞核盐胁迫响应基因的转录本被过度激活。综上所述，拟南芥CYSTM3在盐胁迫耐受性中起负调控作用。
CYSTM3, a small mitochondrial protein, acts as a negative regulator in salt stress response by preventing Na+ efflux and disturbing reactive oxygen species (ROS) homeostasis in Arabidopsis. Cysteine-rich transmembrane module (CYSTM) is a not well characterized small peptide family in plants. In this study, we identified a novel mitochondrion-localized CYSTM member CYSTM3 from Arabidopsis, which was ubiquitously expressed in different tissues and dramatically induced by salt stress. Transgenic plants overexpressing CYSTM3 (OE) displayed hypersensitivity to salt stress compared with wild type (WT) plants, whereas a knockout mutant cystm3 was more tolerant to high salinity than WT. Moreover, OE lines accumulated higher contents of Na+ and ROS than WT and cystm3 upon exposure to high salinity. Further analysis revealed that CYSTM3 could deter root Na+ efflux and inhibit the activities of a range of ROS scavenging enzymes in Arabidopsis. In addition, the transcripts of nuclear salt stress-responsive genes were over-activated in cystm3 than those in WT and OE lines. Taken together, Arabidopsis CYSTM3 acts as a negative regulator in salt stress tolerance.