Phage display selection of peptides that inhibit metastasis ability of gastric cancer cells with high liver-metastatic potential
Phage display selection of peptides that inhibit metastasis ability of gastric cancer cells with high liver-metastatic potential
复制标题
噬菌体展示了抑制具有高肝转移潜力的胃癌细胞的转移能力的肽的选择。
DOI：
10.1016/j.bbrc.2006.01.047
复制
发表时间：
2006-03-24
影响因子：
3.1
通讯作者：
Fan, DM
中科院分区：
文献类型：
--
作者：
Hu, SJ;Guo, XN;Fan, DM
器官特异性转移是癌细胞的重要特征。肿瘤细胞可以转移到特定的器官，其细胞膜上的蛋白质可能具有潜在的诊断标志物和治疗靶点。本研究以胃癌肝转移细胞为研究对象，利用噬菌体展示肽库技术，成功筛选出与胃癌肝转移细胞结合的8肽配体。噬菌体20对XGC 9811 L细胞的结合效率最高，且具有明显的细胞特异性。噬菌体20展示的肽20能显著抑制XGC 981-L的运动和侵袭能力。XGC 9811-L与胶原W的粘附能力也被肽20抑制。phage 20能显著降低胃癌裸鼠移植瘤的肝转移发生率，并有利于减少肝内转移结节的数量。因此，噬菌体展示技术是筛选与器官特异性转移相关的新分子的有效方法。筛选出的多肽20可以逆转胃癌细胞的肝转移行为。(c)2006爱思唯尔公司All rights reserved.
Organ-specific metastasis is an important character of cancer cells. Cancer cells that can metastasize to a special organ were thought to have different proteins in cell membrane, which might have potential utility as diagnostic markers and therapeutic targets. In the present work, based on high liver-metastatic gastric cancer cells, XGC9811-L, a screening approach with phage displayed peptide library, was successfully used to isolate 8-mer peptide ligands binding to the target cells. The phage20 had the highest binding efficiency to XGC9811L cells, which also displayed remarkable cell specificity. Peptide20 that was displayed on phage20 could suppress the motility and invasion of XGC981-L significantly. The adhesive ability of XGC9811-L to collagen W was also inhibited by peptide20. Furthermore, phage20 could significantly reduce the incidence of liver metastasis of gastric cancer transplanted into nude mice and was also beneficial for the reduction the number of metastatic nodles in the liver. In conclusion, the phage display is an effective method to screen for the new molecules associated with organ-specific metastasis. The selected peptide20 can reverse the liver metastasis behavior of the gastric cancer cells. (c) 2006 Elsevier Inc. All rights reserved.