EXPRESSION ANALYSIS OF GREEN FLUORESCENT PROTEIN IN RETINAL NEURONS OF FOUR TRANSGENIC MOUSE LINES
EXPRESSION ANALYSIS OF GREEN FLUORESCENT PROTEIN IN RETINAL NEURONS OF FOUR TRANSGENIC MOUSE LINES
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DOI：
10.1016/j.neuroscience.2009.01.081
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发表时间：
2009-04-21
期刊：
影响因子：
3.3
通讯作者：
Waessle, H.
中科院分区：
文献类型：
--
作者：
Haverkamp, S.;Inta, D.;Waessle, H.
在细胞特异性启动子控制下表达增强型绿色荧光蛋白（EGFP）的转基因小鼠已被成功用于识别和标记视网膜的特异性细胞类型。我们利用四种转基因小鼠品系研究了EGFP在小鼠视网膜中的表达。在无长突细胞、移位的无长突细胞和神经节细胞中发现了由钙视网膜蛋白启动子驱动的EGFP表达。EGFP表达和钙视蛋白免疫标记的比较表明，许多但不是所有的细胞似乎是双标记的。在胆碱乙酰转移酶启动子的控制下的绿色荧光蛋白的表达被发现在无长突细胞，然而，该细胞并不对应于众所周知的小鼠视网膜的胆碱能（星爆）细胞。在小清蛋白启动子的控制下，EGFP的表达仅限于内核层的无长突细胞和神经节细胞层的细胞（移位的无长突细胞和神经节细胞）。大多数细胞也免疫反应的小清蛋白，然而，标记强度的差异进行了观察。由5-HT 3A受体（5-HTR 3A）的启动子驱动的EGFP表达仅限于5型双极细胞。与此相反，5-HTR 3A的免疫染色被发现在突触热点在sublamina 1的内丛状层，是不相关的5型双极细胞。结果表明，这些转基因小鼠是非常有用的未来的电生理研究的特定类型的无长突和双极细胞表达EGFP，从而允许直接微电极靶向显微镜下控制。(C)2009年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Transgenic mice that express enhanced green fluorescent protein (EGFP) under the control of a cell-specific promoter have been used with great success to identify and label specific cell types of the retina. We studied the expression of EGFP in the retina of mice making use of four transgenic mouse lines. Expression of EGFP driven by the calretinin promoter was found in amacrine, displaced amacrine and ganglion cells. Comparison of the EGFP expression and calretinin immunolabeling showed that many but not all cells appear to be double labeled. Expression of EGFP under the control of the choline acetyltransferase promoter was found in amacrine cells; however, the cells did not correspond to the well known cholinergic (starburst) cells of the mouse retina. The expression of EGFP under the control of the parvalbumin promoter was restricted to amacrine cells of the inner nuclear layer and to cells of the ganglion cell layer (displaced amacrine cells and ganglion cells). Most of the cells were also immunoreactive for parvalbumin, however, differences in labeling intensity were observed. The expression of EGFP driven by the promoter for the 5-HT3 A receptor (5-HTR3A) was restricted to type 5 bipolar cells. In contrast, immunostaining for 5-HTR3A was found in synaptic hot spots in sublamina 1 of the inner plexiform layer and was not related to type 5 bipolar cells. The results show that these transgenic mice are very useful for future electrophysiological studies of specific types of amacrine and bipolar cells that express EGFP and thus permit directed microelectrode targeting under microscopic control. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.