Fusobacterium nucleatum interaction with Pseudomonas aeruginosa induces biofilm-associated antibiotic tolerance via FadA
Fusobacterium nucleatum interaction with Pseudomonas aeruginosa induces biofilm-associated antibiotic tolerance via FadA
复制标题
具核梭杆菌与铜绿假单胞菌相互作用通过 FadA 诱导生物膜相关抗生素耐受
作者：
Li Qian;Tan Lisi;Wang Hongyan;Kou Yurong;Shi Xiaoting;Zhang Shuwei;Pan Yaping
铜绿假单胞菌或具核梭杆菌的呼吸道感染与慢性阻塞性肺疾病急性加重（AECOPD）和抗生素治疗失败相关。然而，这些双物种相互作用对COPD严重程度和生物膜抗生素敏感性的影响仍然知之甚少。本研究表明F.呼吸道内具核梭菌与铜绿假单胞菌常共存，且具核梭菌数量较多。AECOPD患者的肺功能与核仁呈负相关。铜绿假单胞菌与F.具核质通过形成致密的生物被膜促进细菌增殖，并通过过量的Pel和Psl多糖诱导抗生素耐受。此外，F.具核粘附素FadA，而不是其废培养基，诱导铜绿假单胞菌生物膜的抗生素耐受性。这些结果表明F. nucleatum是AECOPD患者肺功能下降的生物标志物，在体外与铜绿假单胞菌通过FadA相互作用产生抗生素抗性，可能成为此类双菌种感染的潜在抗感染靶点。
Respiratory infection with Pseudomonas aeruginosa or Fusobacterium nucleatum is associated with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and failure in antibiotic treatment. However, the impact of these dual-species interactions on the severity of COPD and biofilm antibiotic susceptibility remains poorly understood. This study demonstrated that F. nucleatum frequently coexisted with P. aeruginosa in the respiratory tract, and the number of F. nucleatum was negatively correlated with lung function of AECOPD patients. Coculture of P. aeruginosa and F. nucleatum promoted bacterial proliferation, and induced antibiotic tolerance through forming a dense biofilm surrounding by excessive Pel and Psl polysaccarides. Moreover, F. nucleatum adherin FadA, rather than its spent medium, induced antibiotic tolerance of P. aeruginosa biofilm. These results indicate that F. nucleatum is a biomarker of lung function decline in AECOPD patients, and in vitro interacts with P. aeruginosa to resist antibiotics via FadA, which would be a potential anti-infective target of these dual-species infection.