Cryogenic Fluorescence Spectroscopy of Ionic Fluorones in Gaseous and Condensed Phases: New Light on Their Intrinsic Photophysics.
Cryogenic Fluorescence Spectroscopy of Ionic Fluorones in Gaseous and Condensed Phases: New Light on Their Intrinsic Photophysics.
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DOI：
10.1021/acs.jpca.2c07231
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发表时间：
2022-12
期刊：
影响因子：
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通讯作者：
Eleanor K Ashworth;J. Langeland;M. Stockett;T. T. Lindkvist-T.;Christina Kjær;J. Bull;S. Nielsen
中科院分区：
文献类型：
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作者：
Eleanor K Ashworth;J. Langeland;M. Stockett;T. T. Lindkvist-T.;Christina Kjær;J. Bull;S. Nielsen
通过电喷雾电离产生的气相离子的荧光光谱是一种新兴技术，能够直接探测内在分子光物理，而不受溶剂相互作用的干扰。虽然气相荧光技术的持续发展还有很大的空间，但最近扩展到低温操作条件，可以获得大量有关本征荧光团光物理学的数据，与室温测量相比，提供了更高的光谱分辨率，并且没有阻碍激发态动力学的基质效应。本视角回顾了目前在理解阴离子荧光酮染料的光物理学方面取得的进展，阴离子荧光酮染料在气相中表现出异常大的斯托克斯位移，并讨论了气相和凝聚相荧光光谱的比较如何能够指纹结构动力学。荧光发射和激发光谱的温度依赖性测量能力有助于为使用荧光酮染料作为大分子结构测定中的荧光标签奠定基础。我们提出技术开发的想法。
Fluorescence spectroscopy of gas-phase ions generated through electrospray ionization is an emerging technique able to probe intrinsic molecular photophysics directly without perturbations from solvent interactions. While there is ample scope for the ongoing development of gas-phase fluorescence techniques, the recent expansion into low-temperature operating conditions accesses a wealth of data on intrinsic fluorophore photophysics, offering enhanced spectral resolution compared with room-temperature measurements, without matrix effects hindering the excited-state dynamics. This perspective reviews current progress on understanding the photophysics of anionic fluorone dyes, which exhibit an unusually large Stokes shift in the gas phase, and discusses how comparison of gas- and condensed-phase fluorescence spectra can fingerprint structural dynamics. The capacity for temperature-dependent measurements of both fluorescence emission and excitation spectra helps establish the foundation for the use of fluorone dyes as fluorescent tags in macromolecular structure determination. We suggest ideas for technique development.