IKK-i, a novel lipopolysaccharide-inducible kinase that is related to IκB kinases
IKK-i, a novel lipopolysaccharide-inducible kinase that is related to IκB kinases
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DOI：
10.1093/intimm/11.8.1357
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发表时间：
1999-08-01
影响因子：
4.4
通讯作者：
Akira, S
中科院分区：
文献类型：
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作者：
Shimada, T;Kawai, T;Akira, S
利用抑制性消减杂交技术，我们在小鼠巨噬细胞系RAW 264.7中分离到一个新的激酶IKK-1，它的信息被脂多糖（LPS）强烈诱导。预测的蛋白质在其N-末端含有激酶结构域，与IKK-α或IKK-β的结构域有30%的同源性，C-末端部分含有亮氨酸拉链和潜在的螺旋-环-螺旋结构域，如IKK-α和IKK-β的情况。IKK-i主要在免疫细胞中表达，并且除了LPS之外，还响应于促炎细胞因子如肿瘤坏死因子-α、IL-1和IL-6而被诱导。野生型IKK-1的过表达使I κ B-α的丝氨酸残基Ser 32和Ser 36磷酸化（优先为Ser 36），并显著刺激NF-κ B活化。这些结果表明IKK-1是一种诱导型I κ B激酶，可能在免疫应答中发挥特殊作用。
Using the suppression subtractive hybridization technique, we isolated a novel kinase, IKK-i, whose message is drastically induced by lipopolysaccharide (LPS) in the mouse macrophage cell line RAW264.7, The predicted protein contains the kinase domain in its N-terminus, which shares 30% identity to that of IKK-alpha or IKK-beta, The C-terminal portion contains a leucine zipper and a potential helix-loop-helix domain, as in the case of IKK-alpha and IKK-beta. IKK-i is expressed mainly in immune cells, and is induced in response to proinflammatory cytokines such as tumor necrosis factor-alpha, IL-1 and IL-6, in addition to LPS. Overexpression of wild-type IKK-i phosphorylated serine residues Ser32 and Ser36 of I kappa B-alpha (preferentially Ser36), and significantly stimulated NF-kappa B activation. These results suggest that IKK-i is an inducible I kappa B kinase which may play a special role in the immune response.