An injectable chitosan/chondroitin sulfate hydrogel with tunable mechanical properties for cell therapy/tissue engineering
An injectable chitosan/chondroitin sulfate hydrogel with tunable mechanical properties for cell therapy/tissue engineering
复制标题
DOI：
10.1016/j.ijbiomac.2018.02.069
复制
发表时间：
2018-07-01
影响因子：
8.2
通讯作者：
Lerouge, Sophie
中科院分区：
文献类型：
--
作者：
Alinejad, Yasaman;Adoungotchodo, Atma;Lerouge, Sophie
壳聚糖（Chitosan，CH）水凝胶是近年来通过碳酸氢钠（sodium hydrogen carbonate，SHC）与另一种弱碱如β-甘油磷酸盐（beta-glycerophosphate，BGP）结合而合成的一种具有优异力学性能和快速凝胶化速率的水凝胶。为了改善它们的生物反应，在本研究中，硫酸软骨素（CS）被添加到这些CH水凝胶。水凝胶的pH值和渗透压，以及流变学，机械，形态学和溶胀性能方面的特性，进行了研究，在CS的存在和不存在。通过评价包封的L929成纤维细胞的活力和代谢活性，还评估了CS添加对水凝胶的细胞相容性的影响。新的CH水凝胶含有CS的热敏性和注射的pH值和渗透压接近生理水平和增强的溶胀能力。包封的细胞能够保持其活力和增殖能力长达7天，CS的加入提高了细胞的活力，特别是在无血清条件下。CS的加入显示出对完全凝胶化后的水凝胶的机械强度的降低和剂量依赖性作用。这项工作提供的证据表明，CH-CS水凝胶与SHC和BGP作为胶凝剂的组合制备具有很好的潜力，可用作热敏性，可注射性和生物相容性矩阵与可调的机械性能的细胞治疗应用。(C)2018爱思唯尔B. V.保留所有权利。
Chitosan (CH) hydrogels with remarkable mechanical properties and rapid gelation rate were recently synthesized by combining sodium hydrogen carbonate (SHC) with another weak base, such as beta-glycerophosphate (BGP). To improve their biological responses, in the present study, chondroitin sulfate (CS) was added to these CH hydrogels. Hydrogel characteristics in terms of pH and osmolarity, as well as rheological, mechanical, morphological and swelling properties, were studied in the absence and presence of CS. Effect of CS addition on cytocompatibility of hydrogels was also assessed by evaluating the viability and metabolic activity of encapsulated L929 fibroblasts. New CH hydrogels containing CS were thermosensitive and injectable with pH and osmolality close to physiological levels and enhanced swelling capacity. Encapsulated cells were able to maintain their viability and proliferative capacity up to 7 days and CS addition improved the viability of the cells, particularly in serum-free conditions. Addition of CS showed a reducing and dose-dependent effect on the mechanical strength of the hydrogels after complete gelation. This work provides evidence that CH-CS hydrogels prepared with a combination of SHC and BGP as a gelling agent have a promising potential to be used as thermosensitive, injectable and biocompatible matrices with tunable mechanical properties for cell therapy applications. (C) 2018 Elsevier B.V. All rights reserved.