Extracts of bone contain a potent regulator of bone formation.
Extracts of bone contain a potent regulator of bone formation.
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骨提取物含有有效的骨形成调节剂。
DOI：
10.1016/0304-4165(82)90123-4
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Baylink,DJ
中科院分区：
文献类型：
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作者：
Drivdahl,RH;Howard,GA;Baylink,DJ
我们制备了鸡胚整个股骨和胫骨的水提取物。含有25 μg蛋白质的量的提取物导致颅骨细胞培养物中DNA合成增加500%，并且在5 μg（55%）时检测到显著效果。刺激DNA合成的时间过程显示，在加入提取物后16-20小时出现峰值。该基质因子是不可透析的，并且在Sephadex G-100柱上的分级分离表明分子量为60-80 000。在所用的最大剂量下，[3 H]脯氨酸掺入到颅骨细胞的总蛋白中增加了55%，并且迄今为止，已经发现在促进DNA合成中具有活性的所有组分都增加了培养鸡胫骨中的胶原合成。这些数据与对成骨细胞以及骨前体细胞的影响一致。从2日龄雏鸡的骨髓中提取的提取物也刺激DNA合成（增加280%），从而排除了该因子是来自造血细胞系的相对非特异性有丝分裂原的可能性。我们的结论是，骨基质中含有一种物质，它可以调节骨形成在体外通过控制成骨前体细胞的有丝分裂和/或刺激成骨细胞的活性。
We prepared aqueous extracts of whole femorae and tibiae of embryonic chicks. An amount of extract containing 25 μg of protein resulted in a 500% increase in DNA synthesis in calvarial cell cultures, and significant effects were detected with 5 μg (55%). The time course for stimulation of DNA synthesis showed a peak occurring 16–20 h after addition of the extract. This matrix factor is nondialyzable, and fractionation on a column of Sephadex G-100 indicated a molecular weight of 60–80 000. At the maximum dose used, [3H]proline incorporation into total protein of calvarial cells was increased by 55%, and thus far, all fractions active in promoting DNA synthesis have been found to increase collagen synthesis in culture chick tibiae. These data are consistent with an effect on osteoblasts as well as bone precursor cells. Extracts prepared from tibiae of 2-day-old chicks, from which the marrow had been removed, also stimulated DNA synthesis (280% increase), thus ruling out the possibility that the factor is a relatively nonspecific mitogen from the hematopoietic cell line. We conclude that bone matrix contains a substance which could regulate bone formation in vitro by control of mitosis in osteogenic precursors and/or stimulation of osteoblast activity.