Quantitative analysis and comparative regional investigation of the extracellular matrix of the porcine temporomandibular joint disc
Quantitative analysis and comparative regional investigation of the extracellular matrix of the porcine temporomandibular joint disc
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DOI：
10.1016/j.matbio.2004.11.006
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发表时间：
2005-02-01
期刊：
影响因子：
6.9
通讯作者：
Athanasiou, KA
中科院分区：
文献类型：
--
作者：
Detamore, MS;Orfanos, JG;Athanasiou, KA
颞下颌关节（TMJ）关节盘细胞外基质的特性对于推进关节盘组织工程的研究至关重要。然而，当前文献在描述TMJ关节盘基质的性质方面并不完整，且常常相互矛盾。本研究的目的是利用酶联免疫吸附测定（ELISA）对这些基质成分进行定量，通过免疫组织化学确定其区域分布，并使用扫描电子显微镜（SEM）检查胶原纤维的直径和方向，从而确定猪关节盘三个轴向上关键基质成分的变化。TMJ关节盘的总糖胺聚糖（GAG）含量（包括硫酸皮肤素蛋白聚糖）为干重的5.3±1.2%。硫酸软骨素占总GAG含量的74%，其含量分别是硫酸皮肤素蛋白聚糖、硫酸角质素和透明质酸的4.4倍、8.2倍和164倍。一般来说，这些GAG在TMJ关节盘的中间带最为集中，呈密集簇状分布，在后带最不集中。此外，硫酸软骨素在内侧比外侧更为丰富。Ⅱ型胶原被发现有微量存在，在中间带相对含量较高。观察到胶原纤维主要呈环状围绕关节盘周边分布，并在中间带前后向分布，平均纤维直径为18±9μm。对TMJ关节盘的特性研究，包括先前的生物力学和细胞研究以及当前对细胞外基质的研究，共同揭示了关节盘中间带与其前带和后带相比具有独特的特征。（c）2004爱思唯尔B.V/国际基质生物学学会。版权所有。
Characterization of the extracellular matrix of the temporomandibular joint (TMJ) disc is crucial to advancing efforts in tissue engineering the disc. However, the current literature is incomplete and often contradictory in its attempts to describe the nature of the TMJ disc matrix. The aim of this study was to identify the variation of key matrix components along the three axes of the porcine disc using EUSAs to quantify these matrix components, immunohistochemistry to identify their regional distribution, and SEM to examine collagen fiber diameter and orientation. The overall GAG content of the TMJ disc (including the dermatan sulfate proteoglycans) was 5.3 +/- 1.2% of the dry weight. Chondroitin sulfate, which comprised 74% of this total GAG content, was 4.4, 8.2, and 164 times more abundant than dermatan sulfate proteoglycan, keratan sulfate, and hyaluronic acid, respectively. In general, these GAGs were most concentrated in the intermediate zone of the TMJ disc, appearing in dense clusters, and least concentrated in the posterior band. Additionally, chondroitin sulfate was more abundant medially than laterally. Collagen II was discovered in trace amounts, with higher relative amounts in the intermediate zone. Collagen fibers were observed to run primarily in a ring-like fashion around the periphery of the disc and anteroposteriorly through the intermediate zone, with a mean fiber diameter of 18 +/- 9 mu m. Characterization studies of the TMJ disc, including prior biomechanical and cell studies along with the current study of the extracellular matrix, collectively reveal a distinct character of the intermediate zone of the disc compared to its anterior and posterior bands. (c) 2004 Elsevier B.V/International Society of Matrix Biology. All rights reserved.