Cooperation of EB1-Mal3 and the Bub1 spindle checkpoint
Cooperation of EB1-Mal3 and the Bub1 spindle checkpoint
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DOI：
10.4161/cc.5.1.2262
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发表时间：
2006-01-01
期刊：
影响因子：
4.3
通讯作者：
Toda, T
中科院分区：
文献类型：
--
作者：
Asakawa, K;Toda, T
EB1是一种保守的微管结合蛋白，优先定位于微管正端的生长尖端。虽然EB1在细胞极性控制中的功能已经确立，但其在有丝分裂中的作用仍然难以捉摸。在这里，我们讨论了Mal3在有丝分裂过程和染色体稳定性中的作用，Mal3是分裂酵母EB1的同源物。我们的分析揭示了Mal3对双极微管附着的要求及其与主轴装配检查点的合作作用。特别是Mal3与这个检查点途径的一个特定分支合作，这个分支依赖于Bub1，而不是Mad2。在某些类型的人类癌症中，eb1介导的通路的故障可能与染色体不稳定直接相关，因此对Mal3有丝分裂作用的新认识将与理解这些癌细胞中的分子缺陷直接相关。
EB1 is a conserved microtubule binding protein that preferentially localizes to the growing tips of the microtubule plus end. Whilst the function of EB1 in cell polarity control is well established, its role during mitosis remains largely elusive. Here we discuss our recent work of roles for Mal3, the fission yeast EB1 homologue, in mitotic progression and chromosome stability. Our analysis sheds light upon the requirement of Mal3 for bipolar microtubule attachment and its cooperative role with the spindle assembly checkpoint. In particular Mal3 collaborates with a specific branch of this checkpoint pathway, which is dependent upon Bub1, but not Mad2. As malfunctioning of the EB1-mediated pathway might be directly related to chromosome instability in some types of human cancers, a new knowledge of Mal3's mitotic role would be of direct relevance to understanding the molecular defects in these cancer cells.