Absorption spectra of corneas in the far ultraviolet region.
Absorption spectra of corneas in the far ultraviolet region.
复制标题
角膜在远紫外区域的吸收光谱。
DOI：
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发表时间：
1997
影响因子：
4.4
通讯作者：
G. Kalmus
中科院分区：
文献类型：
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作者：
A. Lembares;X. H. Hu;G. Kalmus
目的
目的研究角膜在260 ~ 190 nm远紫外光区的吸收。
方法
用显微切片机-低温恒温器从18个猪角膜和6个人角膜中获得34个厚度接近20微米的角膜样品。作者使用双光束分光光度计，在350至190 nm的两个UV光学窗口的支持下，对切片角膜样本进行吸光度测量。用三个完整的猪角膜研究冷冻对350至290 nm附近吸光度的影响。
结果
本文测量了猪和人角膜在350 ~ 190 nm范围内的吸收光谱，确定了260 ~ 190 nm范围内的三个吸收段。猪角膜的线性吸收系数在210 nm处为2300 +/- 330（cm-1），在193 nm处为2410 +/- 370（cm-1），人角膜的线性吸收系数在210 nm处为2320 +/- 470（cm-1），在193 nm处为2340 +/- 150（cm-1）。
结论
在220和190 nm之间的远UV区域中的“消融窗口”已经被识别，其中相似持续时间和不同波长的短激光脉冲可以互换以消融具有相似特性的角膜表面。
PURPOSE
To study the corneal absorption in the far ultraviolet (UV) region between 260 and 190 nm.
METHODS
Thirty-four corneal samples of thickness near 20 microns were obtained from 18 porcine corneas and six human corneas with a microtome-cryostat. The authors conducted absorbance measurements of the sectioned corneal samples supported by two UV optical windows from 350 to 190 nm using a dual-beam spectrophotometer. Three whole porcine corneas were used to study the effect of freezing on the absorbance from 350 to near 290 nm.
RESULTS
The absorption spectra of porcine and human corneas from 350 to 190 nm were measured and three segments in the spectrum between 260 and 190 nm have been identified. The linear absorption coefficients were determined to be 2300 +/- 330 (cm-1) at 210 nm and 2410 +/- 370 (cm-1) at 193 nm for the porcine corneas and 2320 +/- 470 (cm-1) at 210 nm and 2340 +/- 150 (cm-1) at 193 nm for the human corneas.
CONCLUSIONS
A "window of ablation" in the far UV region between 220 and 190 nm has been identified in which short laser pulses of similar durations and different wavelengths may be interchangeable to ablate the corneal surface with similar characteristics.