Functional expression of the costimulatory molecule, B7/BB1, on murine dendritic cell populations.
Functional expression of the costimulatory molecule, B7/BB1, on murine dendritic cell populations.
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共刺激分子 B7/BB1 在鼠树突状细胞群上的功能表达。
DOI：
10.1084/jem.176.4.1215
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发表时间：
1992-10-01
影响因子：
15.3
通讯作者：
Lowry, R P
中科院分区：
文献类型：
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作者：
Larsen, C P;Ritchie, S C;Pearson, T C;Linsley, P S;Lowry, R P
尽管树突状细胞（DC）在体外和体内都是T细胞的有效激活剂，但在DC上表达的关键共刺激分子尚未得到很好的表征。利用免疫细胞化学和分子技术，我们发现脾脏DC表达CD28的对抗受体B7/BB1。此外，在表皮朗格汉斯细胞（LC）功能成熟为强效T细胞刺激物的过程中，B7/BB1的表达上调。在阻断实验中，我们发现在dc驱动的原发性混合白细胞反应中，B7/BB1的参与对于未引物的异体T细胞的最佳增殖是必需的。这些数据表明，DC上B7/BB1的调控表达可能在初始T细胞反应的启动中起重要作用。
Whereas dendritic cells (DC) are known to be potent activators of T cells both in vitro and in vivo, the critical costimulatory molecules expressed on DC are not well characterized. Using immunocytochemical and molecular techniques we find that splenic DC express B7/BB1, the counter-receptor for CD28. Moreover, expression of B7/BB1 is upregulated on epidermal Langerhans cells (LC) during their functional maturation into potent T cell stimulators. In blocking experiments, we find that participation of B7/BB1 is required for optimal proliferation of unprimed, allogeneic T cells in DC-driven, primary mixed leukocyte reactions. These data demonstrate that the regulated expression of B7/BB1 on DC may be important in the initiation of a primary T cell response.