Monoclonal Antibodies as Tools to Analyze the Serological and Genetic Complexities of Major Transplantation Antigens
Monoclonal Antibodies as Tools to Analyze the Serological and Genetic Complexities of Major Transplantation Antigens
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单克隆抗体作为分析主要移植抗原的血清学和遗传复杂性的工具
DOI：
10.1111/j.1600-065x.1979.tb00292.x
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发表时间：
1979
影响因子：
8.7
通讯作者：
C. Milstein
中科院分区：
文献类型：
--
作者：
J. Howard;G. Butcher;G. Galfré;C. Milstein;C. Milstein
大鼠MHC类似于其他物种的MHC，表现出多种MHC特征性功能的广泛多态性：通过血清学测定检测的抗原，通过细胞测定检测的抗原，如MLR、GVH和CML，以及多种细胞和非细胞抗原的免疫应答基因（Gunther & Stark 1977，Gasser 1977）。然而，由于缺乏实验室重组体，对该地区的遗传结构知之甚少。本研究源于这样的认识：针对复杂多态性抗原的单克隆抗体的高分辨率可以弥补遗传水平上分辨率的不足。给予合适的单克隆同种抗体，由MHC指定的多态性分子的数量和抗原结构原则上可以以比通过使用计划免疫和常规血清的分离的重组体的分析可能的更高的精确度进行检查。本文综述了用同种免疫大鼠脾细胞与小鼠浆细胞瘤细胞融合制备单克隆抗体的方法及其性质分析的结果。一些初步数据已在其他地方发表（Galfre等人，1977年;霍华德等人，1978年）。
The rat MHC resembles that of other species in displaying extensive polymorphism for a variety of MHC-characteristic functions: antigens detected by serological assays, antigens detected by cellular assays such as the MLR, GVH and CML, and immune response genes for a variety of cellular and non-cellular antigens (Gunther & Stark 1977, Gasser 1977). Nevertheless very little is known about the genetic structure of the region because of the shortage of laboratory recombinants. The present study grew out of the realisation that the high resolving power of monoclonal antibodies against complex polymorphic antigens could compensate for lack of resolution at the genetic level. Granted suitable monoclonal alloantibodies, the number and antigenic structure of the polymorphic molecules specified by the MHC can in principle be examined with greater precision than is possible by analysis of recombinants using planned immunizations and absorptions of conventional sera. This review describes the preparation of monoclonal antibodies by fusion of spleen cells from alloimmunized rats with mouse plasmacytoma cells and some results of an analysis of the properties of these antibodies. Some preliminary data have been published elsewhere (Galfre et al. 1977, Howard et al. 1978).