Global monitoring of autumn gene expression within and among phenotypically divergent populations of Sitka spruce (Picea sitchensis)
Global monitoring of autumn gene expression within and among phenotypically divergent populations of Sitka spruce (Picea sitchensis)
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DOI：
10.1111/j.1469-8137.2007.02346.x
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发表时间：
2008-01-01
期刊：
影响因子：
9.4
通讯作者：
Aitken, Sally N.
中科院分区：
文献类型：
--
作者：
Holliday, Jason A.;Ralph, Steven G.;Aitken, Sally N.
针叶树的冷驯化是一个复杂的过程，它的时间和程度反映了当地的适应，并在许多温带和北方树种的纬度梯度上变化很大。尽管针叶树具有重要的生态和经济价值，但对针叶树秋季冷驯化过程中基因表达的全球变化知之甚少。利用跨越物种范围的三个云杉种群和一个包含21 840个独特元素的云杉基因芯片，在秋季对种群内和种群间的基因表达进行了监测。微阵列数据使用实时聚合酶链式反应对选定的基因进行了验证。在夏末和初冬之间，类似数量的基因显著上调(1257)和下调(967)。其中上调的基因包括脱水蛋白、致病相关/抗冻基因、碳水化合物和脂肪代谢基因以及参与信号转导和转录调控的基因。在早秋和晚秋两个时间点的种群间基因芯片杂交结果显示，秋季转录组发生了很大的变化，其中一些可能反映了局部适应。这些结果揭示了针叶树冷驯化的复杂性，突出了一年生植物耐冷性的异同，为这一重要适应过程的功能和遗传学研究提供了坚实的基础。
Cold acclimation in conifers is a complex process, the timing and extent of which reflects local adaptation and varies widely along latitudinal gradients for many temperate and boreal tree species. Despite their ecological and economic importance, little is known about the global changes in gene expression that accompany autumn cold acclimation in conifers.Using three populations of Sitka spruce (Picea sitchensis) spanning the species range, and a Picea cDNA microarray with 21 840 unique elements, within- and among-population gene expression was monitored during the autumn. Microarray data were validated for selected genes using real-time PCR.Similar numbers of genes were significantly twofold upregulated (1257) and downregulated (967) between late summer and early winter. Among those upregulated were dehydrins, pathogenesis-related/antifreeze genes, carbohydrate and lipid metabolism genes, and genes involved in signal transduction and transcriptional regulation. Among-population microarray hybridizations at early and late autumn time points revealed substantial variation in the autumn transcriptome, some of which may reflect local adaptation.These results demonstrate the complexity of cold acclimation in conifers, highlight similarities and differences to cold tolerance in annual plants, and provide a solid foundation for functional and genetic studies of this important adaptive process.