Characterization of Corrinoid Compounds from Edible Cyanobacterium Nostochopsis sp.
Characterization of Corrinoid Compounds from Edible Cyanobacterium Nostochopsis sp.
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DOI：
10.3177/jnsv.58.50
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发表时间：
2012-02-01
影响因子：
1.6
通讯作者：
Watanabe, Fumio
中科院分区：
文献类型：
--
作者：
Hashimoto, Eri;Yabuta, Yukinori;Watanabe, Fumio
通过微生物法测定可食用蓝细菌Nostochossis sp.的维生素B-12含量为140.6 +/- 16.2 μ g/100 g干重。为了评估念珠藻细胞是否含有维生素B-12或无活性的类可啉化合物，从细胞中纯化类可丽素化合物，然后鉴定为假维生素B-12（97.4 +/- 11.8 μ g/100 g干重）和维生素B-12（43.2 +/- 6.0 μ g/100 g干重）。在维生素B-12补充培养基中生长的念珠藻细胞中维生素B-12含量显著增加（254.8 +/-17.6 μ g/100 g干重）。
Vitamin B-12 content of an edible cyanobacterium, Nostochopsis sp. was determined to be 140.6 +/- 16.2 mu g/100 g dry weight by a microbiological method. To evaluate whether the Nostochopsis cells contain vitamin B-12 or inactive corrinoid compounds, corrinoid compounds were purified from the cells and then identified as pseudovitamin B-12 (97.4 +/- 11.8 mu g/100 g dry weight) and vitamin B-12(43.2 +/- 6.0 mu g/100 g dry weight) on the basis of silica gel 60 TLC bioautograms and LC/ESI-MS/MS chromatograms. Vitamin B-12 content was significantly increased in the Nostochopsis cells (254.8 +/- 17.6 mu g/100 g dry weight) grown in the vitamin B-12-supplemented medium.