(dA-dT) dependent inactivation of the DNA template properties by interaction with netropsin and distamycin A.
(dA-dT) dependent inactivation of the DNA template properties by interaction with netropsin and distamycin A.
复制标题
通过与 netropsin 和偏端霉素 A 相互作用，导致 DNA 模板特性的 (dA-dT) 依赖性失活。
DOI：
10.1093/nar/2.3.391
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发表时间：
1975
影响因子：
14.9
通讯作者：
O. Pitra
中科院分区：
文献类型：
--
作者：
U. Wähnert;O. Zimmer;G. Luck;O. Pitra
多肽抗生素netropsin和偏端霉素A对DNA依赖性核酸合成的抑制作用已被证明与模板DNA的碱基组成有关。许多dA-dT含量完全不同的天然DNA以及poly（dI-dC）-poly（dI-dC）、poly（dA-dT）-poly（dA-dT）、poly（dA）- poly（dT）和poly（dG）- poly（dC）已被研究作为DNA和部分RNA聚合酶反应中的模板。最高的结合效率的netropsin存在的（dA-dT）-含有DNA聚合物和不太明显的相互作用与（dI-dC）-含有聚合物所示的熔融和CD光谱行为的复合物完全反映在模板失活。偏端霉素A也是如此。然而，与netropsin相反，抗生素偏端霉素A表现出与聚（dG）-聚（dC）的一些结合趋势。netropsin衍生物的DNA和（dA-dT）-含有聚合物的结合效果表明在复合物形成的肽基团的氢键的重要性。
The inhibitory effect of the polypeptide antibiotics netropsin and distamycin A on DNA dependent nucleic acid synthesis has been shown to be related to the base composition of the template DNA. A number of natural DNA's of quite different dA-dT content as well as poly (dI-dC)-poly (dI-dC), poly (dA-dT)-poly (dA-dT), poly (dA) - poly (dT) and poly (dG) - poly (dC) has been studied as templates in DNA and in part in RNA polymerase reaction. The highest binding efficiency of netropsin existing for (dA-dT) - containing DNA polymers and the less pronounced interaction with the (dI-dC)-containing polymer shown by the melting and CD spectrral behaviour of the complexes are entirely reflected in the template inactivation. The same is evident for distamycin A. However, in contrast to netropsin the antibiotic distamycin A exhibits some binding tendency to poly (dG) - poly (dC). Binding effects of a netropsin derivative to DNA and (dA-dT) -containing polymers suggest the importance of hydrogen bonds of the peptide groups in the complex formation.