Phosphorus Restriction Changes the Expression of Fibroblast Growth Factor 23 and Its Receptors in Laying Hens
Phosphorus Restriction Changes the Expression of Fibroblast Growth Factor 23 and Its Receptors in Laying Hens
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磷限制改变蛋鸡成纤维细胞生长因子23及其受体的表达
DOI：
10.3389/fphys.2020.00085
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发表时间：
2020-02-14
影响因子：
4
通讯作者：
Yang, Xiaojun
中科院分区：
文献类型：
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作者：
Ren, Zhouzheng;Yan, Jiakun;Yang, Xiaojun
膳食磷过量浪费了不可再生的自然资源，并引起了畜牧业的环境问题。我们假设，产蛋鸡不需要大的安全裕度，因为存在的成纤维细胞生长因子23（FGF 23）的饲料磷。试验1选用40周龄海兰褐蛋鸡504只，随机分为7组，每组6个重复，每组12只，试验期15周，试验组饲粮非植酸磷水平分别为0.12%、0.17%、0.22%、0.27%、0.32%、0.37%和0.42%。试验二选用40周龄海兰褐蛋鸡14只，随机分为两组：限磷组（n = 7），非植酸磷含量为0.14%;常规组（n = 7），非植酸磷含量为0.32%，试验期21 d。试验1和试验2对蛋鸡产蛋性能和蛋品质进行了研究。试验2测定了磷的排泄量和生理变化。结果表明，日粮非植酸磷水平对产蛋性能和蛋品质无显著影响（P > 0.05）。试验2中，添加0.14%非植酸磷的蛋鸡的磷排泄量比添加0.32%非植酸磷的蛋鸡降低了52.6%（P < 0.001）。试验2中的产蛋鸡对0.14%非植酸磷日粮的反应表现为：（1）抑制FGF 23 mRNA的表达（57.8%，P < 0.001）和成纤维细胞生长因子受体1（FGFR 1，52.8%，P = 0.012），（2）降低血清FGF 23水平（41.7%，P = 0.011）和磷（3）肾组织FGFR 1 mRNA表达降低（P < 0.001）（66.0%，P = 0.040）和FGFR 4（63.3%，P = 0.012）和肾脏2a型钠磷协同转运蛋白表达减少NPt 2a增加51%，P = 0.025;（4）NPt 2b蛋白表达增加45%，P = 0.032;（5）钙排泄增加22.9%，P
Dietary phosphorus oversupply wastes non-renewable natural resources and raises environmental concerns in animal agriculture. We hypothesized that laying hens do not need large safety margins for dietary phosphorus because of the existence of fibroblast growth factor 23 (FGF23). In experiment 1, a total of 504 Hy-Line Brown laying hens (40-week-old) were randomly assigned to seven diets (for each diet, six replicates of 12 hens), containing 0.12, 0.17, 0.22, 0.27, 0.32, 0.37, and 0.42% non-phytate phosphorus, respectively, for 15 weeks. In experiment 2, a total of 14 Hy-Line Brown laying hens (40-week-old) were randomly assigned to two diets: (1) phosphorus restricted (n = 7) diet containing 0.14% non-phytate phosphorus, and (2) regular phosphorus (n = 7) diet containing 0.32% non-phytate phosphorus, for 21 days. Laying performance and egg quality were investigated in experiments 1 and 2. Phosphorus excretion and physiological changes were determined in experiment 2. It was found that dietary non-phytate phosphorus levels had no effects (P > 0.05) on laying performance and egg quality in either experiment. In experiment 2, laying hens fed 0.14% non-phytate phosphorus had decreased phosphorus excretion (by 52.6%, P < 0.001) when compared to those fed 0.32% non-phytate phosphorus. In response to the 0.14% non-phytate phosphorus diet, laying hens in experiment 2 exhibited: (1) suppressed calvaria mRNA expressions of FGF23 (by 57.8%, P < 0.001) and fibroblast growth factor receptor 1 (FGFR1, by 52.8%, P = 0.012), (2) decreased serum levels of FGF23 (by 41.7%, P = 0.011) and phosphorus (by 40.3%, P < 0.001), (3) decreased kidney mRNA expressions of FGFR1 (by 66.0%, P = 0.040) and FGFR4 (by 63.3%, P = 0.012) and decreased kidney protein expression of type 2a sodium-phosphorus co-transporter (NPt2a, by 51%, P = 0.025), (4) increased duodenum protein expression of NPt2b (by 45%, P = 0.032), and (5) increased excretion of calcium (by 22.9%, P