Bovine brain and pituitary fibroblast growth factors: comparison of their abilities to support the proliferation of human and bovine vascular endothelial cells.
Bovine brain and pituitary fibroblast growth factors: comparison of their abilities to support the proliferation of human and bovine vascular endothelial cells.
复制标题
牛脑和垂体成纤维细胞生长因子：比较其支持人和牛血管内皮细胞增殖的能力。
DOI：
10.1083/jcb.97.6.1677
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发表时间：
1983-12
期刊：
影响因子：
--
通讯作者：
Lirette M
中科院分区：
文献类型：
--
作者：
Gospodarowicz D;Cheng J;Lirette M
比较了脑和垂体成纤维细胞生长因子（FGF）对人脐静脉和牛主动脉弓血管内皮细胞的促有丝分裂作用。脑和垂体FGF都是促有丝分裂的低密度人脐内皮（HUE）细胞培养物保持在纤连蛋白或层粘连蛋白包被的培养皿或生物基质产生的培养细胞，如牛角膜内皮细胞或畸胎瘤细胞系PF-HR-9。在低至0.25 ng/ml的浓度下，FGF触发HUE细胞的增殖，半最大响应为0.55 ng/ml，最佳效果为2.5至5 ng/ml。它比商业化的内皮细胞生长因子制剂的效力高50，000倍，比商业化的垂体FGF制剂的效力高40倍。当在成年牛主动脉内皮细胞的低密度培养物上测试垂体FGF的作用时，观察到类似的结果。当比较脑和垂体FGF在低密度HUE细胞培养物中的活性时，两种促有丝分裂剂都是活性的。为了确认HUE细胞的酸性和中性以及碱性有丝分裂原的脑提取物中的存在，在酸性（4.5）、中性（7.2）和碱性（8.5）pH下提取脑组织。这三种类型的提取物在支持HUE或成年牛主动脉内皮细胞的增殖方面同样有效。当各种提取物吸附在羧甲基Sephadex C-50柱上，中性和碱性提取物吸附后的活性类似于未吸附的提取物。相比之下，在pH 4.5下制备的提取物损失了90-95%的活性，其在含有FGF的吸附级分中被回收。
The mitogenic effects of brain and pituitary fibroblast growth factors (FGF) on vascular endothelial cells derived from either human umbilical vein or bovine aortic arch have been compared. Both brain and pituitary FGF are mitogenic for low density human umbilical endothelial (HUE) cell cultures maintained on either fibronectin- or laminin-coated dishes or on biomatrices produced by cultured cells such as bovine corneal endothelial cells or the teratocarcinoma cell line PF-HR-9. Pituitary FGF triggered the proliferation of HUE cells at concentrations as low as 0.25 ng/ml, with a half-maximal response at 0.55 ng/ml and optimal effect at 2.5 to 5 ng/ml. It was 50,000-fold more potent than commercial preparations of endothelial cell growth factor and 40 times more potent than commercial preparations of pituitary FGF. Similar results were observed when the effect of pituitary FGF was tested on low density cultures of adult bovine aortic endothelial cells. When the activity of brain and pituitary FGF on low density HUE cell cultures was compared, both mitogens were active. To confirm the presence in brain extract of both acidic and neutral, as well as of basic mitogen, for HUE cells, brain tissues were extracted at acidic (4.5), neutral (7.2), and basic (8.5) pH. The three types of extracts were equally potent in supporting the proliferation of either HUE or adult bovine aortic endothelial cells. When the various extracts were absorbed at pH 6.0 on a carboxymethyl Sephadex C-50 column, the neutral and basic extracts had an activity after adsorption similar to that of unadsorbed extracts. In contrast, extracts prepared at pH 4.5 lost 90-95% of their activity which was recovered in the adsorbed fraction containing FGF.