Quantitative fluorescence tomography using a combined tri-modality FT/DOT/XCT system.
Quantitative fluorescence tomography using a combined tri-modality FT/DOT/XCT system.
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DOI：
10.1364/oe.18.007835
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发表时间：
2010-04-12
期刊：
影响因子：
3.8
通讯作者：
Gulsen G
中科院分区：
文献类型：
--
作者：
Lin Y;Barber WC;Iwanczyk JS;Roeck W;Nalcioglu O;Gulsen G
在这项工作中，首次提出了一种完全集成的三模态系统，该系统将荧光，漫射光学和X射线断层扫描（FT/DOT/XCT）结合到同一设置中。该系统的目的是使用多模态成像方法进行定量荧光断层扫描。XCT解剖信息被用作结构先验，而通过DOT测量获得的光学背景异质性信息被用作功能先验。混合系统的性能进行了评估，使用多模态phantomy。特别是，我们表明，一个2.4 mm直径的荧光夹杂物位于一个异质介质中，可以准确地定位与功能的先验信息，虽然荧光团浓度恢复与70%的误差。另一方面，只有当DOT光学背景泛函和XCT结构先验信息都被用来指导和约束FT重建算法时，荧光团浓度才能在8%的误差内准确地恢复。
In this work, a first-of-its-kind fully integrated tri-modality system that combines fluorescence, diffuse optical and x-ray tomography (FT/DOT/XCT) into the same setting is presented. The purpose of this system is to perform quantitative fluorescence tomography using multi-modality imaging approach. XCT anatomical information is used as structural priori while optical background heterogeneity information obtained by DOT measurements is used as functional priori. The performance of the hybrid system is evaluated using multi-modality phantoms. In particular, we show that a 2.4 mm diameter fluorescence inclusion located in a heterogeneous medium can be localized accurately with the functional a priori information, although the fluorophore concentration is recovered with 70% error. On the other hand, the fluorophore concentration can be accurately recovered within 8% error only when both DOT optical background functional and XCT structural a priori information are utilized to guide and constrain the FT reconstruction algorithm.