Multiplex PCR/mass spectrometry screening of biological carcinogenic agents in human mammary tumors
Multiplex PCR/mass spectrometry screening of biological carcinogenic agents in human mammary tumors
复制标题
人类乳腺肿瘤中生物致癌物质的多重PCR/质谱筛查
DOI：
10.1016/j.jcv.2014.07.010
复制
发表时间：
2014-10-01
影响因子：
8.8
通讯作者：
Jin, Qi
中科院分区：
文献类型：
--
作者：
Peng, Junping;Wang, Ting;Jin, Qi
背景：虽然许多研究表明乳腺癌发病机制与病毒感染之间可能存在联系，但病毒在乳腺癌发生中的作用仍存在争议。目的：我们分析了乳腺肿瘤标本中 30 种致癌人乳头瘤病毒 (HPV)、EB 病毒 (EBV)、卡波西肉瘤疱疹病毒 (KSHV) 和 6 种多瘤病毒的流行情况。研究设计：我们分析了 100 例乳腺癌的乳腺标本来自中国陕西省的患者（第 1 组）和 50 名良性乳腺疾病患者（第 2 组）。我们还对患者和 96 名女性献血志愿者（第 3 组）的血液样本中的病毒进行了筛查。结果：分别在 60 名、14 名和 2 名乳腺癌患者中检测到 EBV、默克尔细胞多瘤病毒（MCPyV）和 HPV-18，分别在 16 名和 1 名良性乳腺疾病患者中检测到 EBV 和 MCPyV。 EBV 和 MCPyV 在第 1 组中比第 2 组中更常见（EBV：60.0% vs. 32.0%，p = 0.0012；MCPyV：14.0% vs. 2.0%；p = 0.02）。相反，第1组和第2组、第1组和第3组之间血液样本中EBV和MCPyV的患病率没有差异。通过原位杂交，在恶性乳腺组织中检测到EBV，并且其存在仅限于恶性细胞。结论：我们发现EBV和MCPyV在乳腺癌女性肿瘤中比良性乳腺疾病女性样本中更常见。我们的结果支持EBV在乳腺癌中的病因学作用中国患者的发病机制。 (C) 2014 Elsevier B.V. 保留所有权利。
Background: While many studies have suggested a possible link between breast cancer pathogenesis and infection by viruses, the role of viruses in breast carcinogenesis remains controversial.Objectives: We analyzed the prevalence of 30 oncogenic human papillomaviruses (HPVs), Epstein-Barr virus (EBV), Kaposi's sarcoma herpes virus (KSHV) and six polyomaviruses in breast tumor specimens.Study design: We analyzed breast specimens from 100 breast cancer patients (group 1) and 50 benign breast disease patients (group 2) from Shaanxi Province in China. We also screened for the viruses in blood samples from the patients and 96 female blood donor volunteers (group 3).Results: EBV, Merkel cell polyomavirus (MCPyV) and HPV-18 were detected in 60, 14 and 2 breast cancer patients, respectively, and EBV and MCPyV were detected in 16 and 1 benign breast disease patients, respectively. EBV and MCPyV were more prevalent in group 1 than in group 2 (EBV: 60.0% vs. 32.0%, p = 0.0012; MCPyV: 14.0% vs. 2.0%; p = 0.02). In contrast, there was no difference in the prevalence of EBV and MCPyV in blood samples between group 1 and group 2, group 1 and group 3. EBV was detected in malignant breast tissue and its presence was confined to the malignant cells using in situ hybridization.Conclusions: We found that EBV and MCPyV were more prevalent in the tumors of women with breast cancer than in samples from women with benign breast disease.Our results support an etiologic role for EBV in breast cancer pathogenesis in Chinese patients. (C) 2014 Elsevier B.V. All rights reserved.