Insights into the virulence of oral biofilms: discoveries from proteomics.

Insights into the virulence of oral biofilms: discoveries from proteomics.
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DOI:
10.1586/epr.12.16
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发表时间:
2012-06
影响因子:
3.4
通讯作者:
Hackett M
Hackett M
中科院分区:
生物学3区
文献类型:
--
作者:
Kuboniwa M;Tribble GD;Hendrickson EL;Amano A;Lamont RJ;Hackett M

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本文综述了与牙菌斑和口腔生物学相关的多菌群、生物膜和宿主反应的选定领域的研究进展。重点是最近的研究,其中蛋白质组学方法,特别是那些使用质谱作为读出,在调查中发挥了重要作用。在过去的5-10年中,这种方法从口腔生物学的边缘过渡到主流,就像在生物医学科学的其他领域一样。出于重点和空间的原因,作者没有讨论与改善口腔健康诊断相关的生物标志物研究,因为这些文献本身相当丰富,值得单独处理。在这里,讨论了菌斑成分生物体、生物膜形成、多物种相互作用和宿主-微生物相互作用的全球基因调控研究。对与多物种系统研究相关的蛋白质组学方法的几个方面进行了评述。
This review covers developments in the study of polymicrobial communities, biofilms and selected areas of host response relevant to dental plaque and related areas of oral biology. The emphasis is on recent studies in which proteomic methods, particularly those using mass spectrometry as a readout, have played a major role in the investigation. The last 5–10 years have seen a transition of such methods from the periphery of oral biology to the mainstream, as in other areas of biomedical science. For reasons of focus and space, the authors do not discuss biomarker studies relevant to improved diagnostics for oral health, as this literature is rather substantial in its own right and deserves a separate treatment. Here, global gene regulation studies of plaque-component organisms, biofilm formation, multispecies interactions and host–microbe interactions are discussed. Several aspects of proteomics methodology that are relevant to the studies of multispecies systems are commented upon.
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