Gestational diabetes mellitus affects the fucosylation and sialylation levels of N/O-glycans in human milk glycoproteins.
Gestational diabetes mellitus affects the fucosylation and sialylation levels of N/O-glycans in human milk glycoproteins.
复制标题
DOI：
10.1016/j.carbpol.2022.120312
复制
发表时间：
2022-11
影响因子：
11.2
通讯作者：
Xiaoqin Wang;Zhenhua Li;Wenqing Li;Cheng Li;J. Liu;Yu Lu;Jiangbo Fan;Haiyan Ren;Linjuan Huang;Zhongfu Wang
中科院分区：
文献类型：
--
作者：
Xiaoqin Wang;Zhenhua Li;Wenqing Li;Cheng Li;J. Liu;Yu Lu;Jiangbo Fan;Haiyan Ren;Linjuan Huang;Zhongfu Wang
妊娠期糖尿病（GDM）对母亲和后代的负面影响，可能与乳蛋白的糖基化水平有关。本文采用HILIC-MS/MS分析了健康人和GDM个体的人乳N/O-糖组，共检测到56种N-糖，其中12种N-糖在GDM和健康人的乳汁中存在显著差异。共检测到25种O-聚糖，其中11种在GDM和健康牛奶中差异较大，尤其是H1N1 S1和H2 N2 S1。总体而言，GDM乳中N/O-聚糖的相对含量显著低于健康乳。GDM乳中岩藻糖基化N-聚糖的比例较高，唾液酸化O-聚糖的比例较低。这些发现将为深入研究milkN/O-聚糖的构效关系提供基础，并有望推动新生儿配方奶粉的设计。
Gestational diabetes mellitus (GDM) has negative effects on mothers and offspring, which may be related to the glycosylation level of milk proteins. Here, the human milkN/O-glycome of healthy and GDM individuals was analyzed by HILIC-MS/MS. A total of 56 putativeN-glycans were detected, among which 12N-glycans were significantly different between GDM and healthy milk. A total of 25 putativeO-glycans were detected, and 11 of them varied greatly between GDM and healthy milk, especially H1N1S1 and H2N2S1. Overall, the relative content ofN/O-glycans in GDM milk was significantly lower than that of healthy milk. In GDM milk, fucosylatedN-glycans present higher proportion, whereas the proportion of sialylatedO-glycans were lower. These findings would provide a foundation for in-depth study on the structure-activity relationship of milkN/O-glycans and are expected to drive the design of infant formula for newborns.