Acidic and basic fibroblast growth factors are survival factors with distinctive activity in quiescent BALB/c 3T3 murine fibroblasts.
Acidic and basic fibroblast growth factors are survival factors with distinctive activity in quiescent BALB/c 3T3 murine fibroblasts.
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酸性和碱性成纤维细胞生长因子是在静止的 BALB/c 3T3 小鼠成纤维细胞中具有独特活性的存活因子。
DOI：
10.1073/pnas.88.8.3372
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发表时间：
1991
影响因子：
11.1
通讯作者：
Zychlinsky,A
中科院分区：
文献类型：
--
作者：
Tamm,I;Kikuchi,T;Zychlinsky,A
血小板源性生长因子 (PDGF)、表皮生长因子和胰岛素样生长因子先前已被确定为对密度抑制的静态 BALB/c 3T3 小鼠成纤维细胞具有独特活性的存活因子。成纤维细胞生长因子 (FGF) 与 PDGF 一样，使静止的 BALB/c 3T3 细胞能够对表皮生长因子和胰岛素样生长因子做出反应，从而介导细胞周期从 G1 进入 S 期 [Stiles, C. D.、Pledger, W. J.、VanWyk, J. J.、Antoniades, H. N. 和 Scher, C. D. (1979) Proc.国家。阿卡德。科学。美国 76, 1279-1283]。我们现在证明 FGF 具有与 PDGF 不同的显着的细胞存活增强活性。酸性 FGF (aFGF) 和碱性 FGF (bFGF) 均显着增强静止细胞的短期（3 小时）存活率。 bFGF 是这两个因子中更活跃的一个，并且单独显示出显着的长期（20 小时）存活促进活性，而 aFGF 需要肝素才能发挥长期活性。 bFGF 或 aFGF 加肝素的保护与细胞周期进入 S 期无关。 aFGF 和 bFGF 的短期（3 小时）和长期（20 小时）保护作用都严重依赖于蛋白质合成，而 PDGF 则不然。积累的证据表明，多种生长因子有助于维持静止小鼠成纤维细胞的完整性，并且它们的作用可能涉及蛋白激酶 A 和 C 介导的过程以及蛋白质合成。不同的生长因子表现出不同的作用模式。
Platelet-derived growth factor (PDGF), epidermal growth factor, and insulin-like growth factor have previously been identified as survival factors with distinctive activities for the density-inhibited quiescent BALB/c 3T3 murine fibroblasts. Fibroblast growth factor (FGF), like PDGF, renders quiescent BALB/c 3T3 cells competent to respond to epidermal growth factor and insulin-like growth factor, which mediate cell-cycle traverse through G1 into S phase [Stiles, C. D., Pledger, W. J., VanWyk, J. J., Antoniades, H. N. & Scher, C. D. (1979) Proc. Natl. Acad. Sci. USA 76, 1279-1283]. We now show that FGF possess marked cell survival-enhancing activity distinctive from that of PDGF. Both acidic FGF (aFGF) and basic FGF (bFGF) markedly enhance short-term (3-hr) survival of quiescent cells. bFGF is the more active of the two factors and shows marked long-term (20-hr) survival-promoting activity alone, whereas aFGF requires heparin for long-term activity. Protection by bFGF or aFGF plus heparin is not associated with cell-cycle traverse into S phase. Both the short-term (3-hr) and long-term (20-hr) protective actions of aFGF and bFGF critically depend on protein synthesis, whereas those of PDGF do not. The accumulated evidence shows that several growth factors can contribute to maintenance of the integrity of quiescent murine fibroblasts and that their action can involve protein kinase A- and C-mediated processes as well as protein synthesis. Different growth factors display distinctive modes of action.