Discovery of unknown new ponds occupied by the endangered giant water bug Kirkaldyia deyrolli (Hemiptera: Heteroptera: Belostomatidae) by combining environmental DNA and capture surveys
Discovery of unknown new ponds occupied by the endangered giant water bug Kirkaldyia deyrolli (Hemiptera: Heteroptera: Belostomatidae) by combining environmental DNA and capture surveys
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通过结合环境 DNA 和捕获调查,发现了被濒临灭绝的巨型水蝽 Kirkaldyia deyrolli(半翅目:异翅目:Belostomatidae)占据的未知新池塘
DOI:
10.1111/ens.12540
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发表时间:
2023
影响因子:
0.9
通讯作者:
Takahara Teruhiko
中科院分区:
文献类型:
--
作者:
Ogata Shigeki;Nishiwaki Atsuhiro;Yamazoe Kanji;Sugai Kyoko;Takahara Teruhiko
Basic ecological information (e.g. habitat or distribution) is indispensable for conserving endangered species. The giant water bugKirkaldyia deyrolliis an aquatic insect in the large stink bug family (Belostomatidae). It inhabits ponds and is a critically endangered species. In this study, we developed a species‐specific primer–probe set (the cytochromecoxidase I region in mitochondrial DNA) that was used in real‐time polymerase chain reaction (PCR) assays to detectK. deyrollienvironmental DNA (eDNA). Next, using eDNA analysis, we investigated the presence ofK. deyrolliin 89 study ponds (including one pond that had already been identified as the habitat of this species) in Shimane Prefecture, Japan. The eDNA ofK. deyrolliwas detected in 11 of these 89 ponds. Furthermore, when the traditional method of direct capture survey was carried out in four of the 10 ponds whereK. deyrollieDNA had been detected and no prior occupancy information for this species was available, the capture of oneK. deyrolli(female) was successful in only one pond. This study showed that combining eDNA analysis and direct capture methods can lead to discovering previously unknown habitats essential for conserving an endangered species.
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影响因子:
0.8
作者:
T. Yoon;Dong Gun Kim;Seon Yi Kim;Shin;Y. Bae
通讯作者:
Y. Bae
影响因子:
1.7
作者:
S. Ohba;H. Miyasaka;F. Nakasuji
通讯作者:
F. Nakasuji
影响因子:
0.9
作者:
S. Ohba
通讯作者:
S. Ohba
DOI:
--
发表时间:
2001
期刊:
--
影响因子:
--
作者:
H. Saijo
通讯作者:
H. Saijo
DOI:
10.11257/jjeez.22.93
发表时间:
2011
期刊:
--
影响因子:
--
作者:
伸也 大庭
通讯作者:
伸也 大庭