Discovery of unknown new ponds occupied by the endangered giant water bug Kirkaldyia deyrolli (Hemiptera: Heteroptera: Belostomatidae) by combining environmental DNA and capture surveys

Discovery of unknown new ponds occupied by the endangered giant water bug Kirkaldyia deyrolli (Hemiptera: Heteroptera: Belostomatidae) by combining environmental DNA and capture surveys
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通过结合环境 DNA 和捕获调查,发现了被濒临灭绝的巨型水蝽 Kirkaldyia deyrolli(半翅目:异翅目:Belostomatidae)占据的未知新池塘

DOI:
10.1111/ens.12540
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发表时间:
2023
影响因子:
0.9
通讯作者:
Takahara Teruhiko
Takahara Teruhiko
中科院分区:
农林科学4区
文献类型:
--
作者:
Ogata Shigeki;Nishiwaki Atsuhiro;Yamazoe Kanji;Sugai Kyoko;Takahara Teruhiko

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基本生态信息(例如栖息地或分布)对于保护濒危物种是必不可少的。巨型水蝽Kirkaldyia deyrollii 是大型臭虫科(Belostomatidae)的水生昆虫。它栖息于池塘,是极度濒危物种。在这项研究中,我们开发了一种物种特异性引物探针组(线粒体 DNA 中的细胞色素氧化酶 I 区域),用于实时聚合酶链反应 (PCR) 检测来检测 K。 脱氧环境 DNA (eDNA)。接下来,我们利用 eDNA 分析研究了 K 的存在。 deyrolliin 日本岛根县 89 个研究池塘(包括一个已被确定为该物种栖息地的池塘)。 K 的 eDNA。 在这 89 个池塘中的 11 个中检测到了 deyrolli。此外,当在 10 个池塘中的 4 个进行直接捕获调查的传统方法时,K. deyrollieDNA 已被检测到,并且没有该物种的先前占用信息,即 oneK 的捕获。 deyrolli(雌性)仅在一个池塘中取得了成功。这项研究表明,结合 eDNA 分析和直接捕获方法可以发现以前未知的栖息地,这对于保护濒危物种至关重要。
Basic ecological information (e.g. habitat or distribution) is indispensable for conserving endangered species. The giant water bugKirkaldyia deyrolliis an aquatic insect in the large stink bug family (Belostomatidae). It inhabits ponds and is a critically endangered species. In this study, we developed a species‐specific primer–probe set (the cytochromecoxidase I region in mitochondrial DNA) that was used in real‐time polymerase chain reaction (PCR) assays to detectK. deyrollienvironmental DNA (eDNA). Next, using eDNA analysis, we investigated the presence ofK. deyrolliin 89 study ponds (including one pond that had already been identified as the habitat of this species) in Shimane Prefecture, Japan. The eDNA ofK. deyrolliwas detected in 11 of these 89 ponds. Furthermore, when the traditional method of direct capture survey was carried out in four of the 10 ponds whereK. deyrollieDNA had been detected and no prior occupancy information for this species was available, the capture of oneK. deyrolli(female) was successful in only one pond. This study showed that combining eDNA analysis and direct capture methods can lead to discovering previously unknown habitats essential for conserving an endangered species.
东亚濒临灭绝的湿地昆虫,巨型水蝽 Lethocerus deyrolli(半翅目:Belostomatidae)的光吸引飞行
DOI: 10.1080/01650424.2010.508045
发表时间: 2010
期刊: Aquatic Insects
影响因子: 0.8
作者:
T. Yoon;Dong Gun Kim;Seon Yi Kim;Shin;Y. Bae
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两栖类猎物在日本稻田巨型水蝽若虫的饮食和生长中的作用
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发表时间: 2007
期刊: Population Ecology
影响因子: 1.7
作者:
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DOI: 10.1111/ens.12334
发表时间: 2018
影响因子: 0.9
作者:
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DOI: --
发表时间: 2001
期刊: --
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发表时间: 2011
期刊: --
影响因子: --
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伸也 大庭
通讯作者: 伸也 大庭