found in neurons, a third member of the Drosophila elav gene family, encodes a neuronal protein and interacts with elav
found in neurons, a third member of the Drosophila elav gene family, encodes a neuronal protein and interacts with elav
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DOI：
10.1016/s0925-4773(02)00444-6
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发表时间：
2003-03
影响因子：
2.6
通讯作者：
M. Samson;Fabienne Chalvet
中科院分区：
文献类型：
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作者：
M. Samson;Fabienne Chalvet
Elav是果蝇神经元分化和维持所必需的基因，编码参与转录后调控的一系列保守蛋白的原型。我们发现在神经元(FNE)中发现了一种编码新的ELAV类似物的基因。我们发现FNE在体外能与RNA结合。FNE转录本存在于整个发育过程中，并含有较长的未翻译区域。在胚胎发育过程中，转录物和蛋白质仅限于中枢神经系统和三叉神经节的神经元。这些功能让人联想到elav。然而，与ELAV相比，FNE的表达延迟，FNE蛋白呈胞质表达，而ELAV呈核表达。GAL4诱导的FNE在神经元中的过表达导致FNE和ELAV内源性基因座产生的稳定转录减少，表明FNE自我调节，也调节ELAV。
elav, a gene necessary for neuronal differentiation and maintenance in Drosophila, encodes the prototype of a family of conserved proteins involved in post-transcriptional regulation. We identified found in neurons (fne), a gene encoding a new ELAV paralogue. We showed that FNE binds RNA in vitro. fne transcripts are present throughout development and contain long untranslated regions. Transcripts and proteins are restricted to neurons of the CNS and PNS during embryogenesis. These features are reminiscent of elav. However, fne expression is delayed compared to elav's, and FNE protein appears cytoplasmic, while ELAV is nuclear. GAL4-directed overexpression of fne in neurons leads to a reduction of stable transcripts produced from both the fne and elav endogenous loci, suggesting that fne autoregulates and also regulates elav.