Cytotoxity of nanoparticles is influenced by size, proliferation and embryonic origin of the cells used for testing
Cytotoxity of nanoparticles is influenced by size, proliferation and embryonic origin of the cells used for testing
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DOI：
10.3109/17435390.2011.586478
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发表时间：
2012-06-01
期刊：
影响因子：
5
通讯作者：
Pieber, Thomas R.
中科院分区：
文献类型：
--
作者：
Froehlich, Eleonore;Meindl, Claudia;Pieber, Thomas R.
细胞毒性筛选是药物化合物筛选中用于鉴定不良细胞效应的常用技术。纳米颗粒可能会干扰这些测定。对于细胞毒性数据的解释，重要的是研究其他因素的影响，如纳米颗粒的预处理，细胞培养基的选择和用于测试的细胞类型。羧基聚苯乙烯颗粒（CPS，20-1000 nm）的物理化学特性和细胞毒性评估与7个筛选试验在20个细胞系，不同的物种，生长模式，细胞大小，倍增时间，胚胎起源和吞噬能力。小CPS在所有细胞系中表现出更强的细胞毒性，大CPS仅在吞噬细胞中表现出更强的细胞毒性。在筛选试验之间观察到细胞毒性的微小差异。细胞的生长方式和细胞大小决定细胞的毒性，而不是细胞的增殖速度和胚胎来源。非贴壁细胞、间充质来源的细胞和具有高增殖率的细胞可能更容易受到纳米颗粒的损伤。
Cytotoxicity screening is a common technique in drug compound screening for the identification of adverse cellular effects. Nanoparticles may cause interference in these assays. For the interpretation of cytotoxicity data it is important to study also the influence of other factors like pre-treatment of the nanoparticles, the choice of the cell culture medium and type of cell used for testing. Carboxyl polystyrene particles (CPS, 20-1000 nm) were physicochemically characterized and cytotoxicity assessed with seven screening assays in 20 cell lines, which differed in species, growth pattern, cell size, doubling time, embryonic origin and capacity for phagocytosis. Small CPS acted more cytotoxic in all cell lines, larger CPS only in phagocytic cells. Small differences in cytotoxicity were noted between the screening assays. Growth pattern and cell size determined cytotoxicity more than proliferation rate and embryonic origin of cells. Non-adherent cells, cells of mesenchymal origin and with high proliferation rate may be more susceptible to damage by nanoparticles.