Identification of side population cells in human hepatocellular carcinoma cell lines with stepwise metastatic potentials
Identification of side population cells in human hepatocellular carcinoma cell lines with stepwise metastatic potentials
复制标题
人肝癌细胞系中具有逐步转移潜力的侧群细胞的鉴定
DOI：
10.1007/s00432-008-0407-1
复制
发表时间：
2008-11-01
影响因子：
3.6
通讯作者：
Wu, Zhi-Quan
中科院分区：
文献类型：
--
作者：
Shi, Guo-Ming;Xu, Yang;Wu, Zhi-Quan
方法采用流式细胞仪从4株具有阶梯转移潜能的肝癌细胞系HCCLM 3、MHCC 97-H、MHCC 97-L和Hep 3B中分选SP细胞，并进行分化、克隆形成、耐药和体内致瘤性分析。结果HCCLM 3、MHCC 97-H、MHCC 97-L、Hep 3B细胞中SP细胞比例分别为28.7 ± 1.6%、14.5 ± 0.6%、4.2 ± 0.4%、0.9 ± 0.1%，差异有统计学意义（P< 0.01）;所有SP细胞均具有自我更新、克隆形成能力强、耐药性强和ABCG 2高表达等特点。低至2，000个SP细胞可在非肥胖糖尿病/严重联合免疫缺陷小鼠中成功地引发肿瘤。结论从HCC细胞系中纯化的SP细胞具有癌干细胞样特性，并可能与HCC的转移潜能和治疗抗性有关。
PurposeTo identify the side population (SP) cells from four hepatocellular carcinoma (HCC) cell lines with stepwise metastatic potentials.MethodsSP cells were sorted from HCCLM3, MHCC97-H, MHCC97-L and Hep3B by flow cytometry, and then analyzed by differentiation study, clonogenic assay, chemoresistance study and tumorigenicity assay in vivo. The expression of ABCG2in SP cells was detected by immunocytochemistry, western blotting and real-time quantitative PCR, respectively.ResultsThere was significant difference in SP proportion among HCCLM3, MHCC97-H, MHCC97-L and Hep3B (28.7 ± 1.6%, 14.5 ± 0.6%, 4.2 ± 0.4%, 0.9 ± 0.1%, respectively,P< 0.01). All the SP cells showed similar characteristics of self-renewal, high clonogenicity, remarkable chemo-resistance and high expression of ABCG2. As low as 2,000 SP cells could initiate tumors in non-obese diabetic/severe combined immunodeficiency mice successfully.ConclusionsSP cells purified from HCC cell lines harbors cancer stem cell-like properties, and may be related to the metastatic potentials and therapeutic-resistance of HCC.