Increased Levels of Cell-Free Human Placental Lactogen mRNA at 28-32 Gestational Weeks in Plasma of Pregnant Women With Placenta Previa and Invasive Placenta
Increased Levels of Cell-Free Human Placental Lactogen mRNA at 28-32 Gestational Weeks in Plasma of Pregnant Women With Placenta Previa and Invasive Placenta
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DOI：
10.1177/1933719113492209
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发表时间：
2014-02-01
影响因子：
2.9
通讯作者：
Mizumoto, Yoshifumi
中科院分区：
文献类型：
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作者：
Kawashima, Akihiro;Sekizawa, Akihiko;Mizumoto, Yoshifumi
我们比较了在妊娠28 - 32周时，诊断为前置胎盘或侵袭性胎盘的妇女和正常妊娠妇女血浆中无细胞人胎盘乳原（hPL）信使RNA （mRNA）的水平。进一步探讨hPL mRNA预测侵袭性胎盘的敏感性和特异性。采用实时逆转录酶聚合酶链反应对前置胎盘（n = 13）、侵袭性胎盘（n = 5）和正常妊娠（n = 92）妇女的血浆hPL mRNA进行定量。前置胎盘组hPL mRNA中位数（范围）显著高于正常妊娠组，为782（10-2301）拷贝/mL血浆，侵袭性胎盘组为615（522-2102）拷贝/mL血浆，而正常妊娠组为90（4-4407）拷贝/mL血浆，P < 0.01和P < 0.05。我们发现预测前置胎盘女性浸润性胎盘的敏感性为100%，特异性为61.5%。综上所述，妊娠28 ~ 32周时前置胎盘和侵袭性胎盘患者血浆中hPL mRNA表达升高。
We compared the levels of cell-free human placental lactogen (hPL) messenger RNA (mRNA) in maternal plasma at 28 to 32 weeks of gestation between women with diagnosis of placenta previa or invasive placenta and women with an uneventful pregnancy. Sensitivity and specificity of hPL mRNA for the prediction of invasive placenta were further explored. Plasma hPL mRNA were quantified by real-time reverse-transcriptase polymerase chain reaction in women with placenta previa (n = 13), invasive placenta (n = 5), and normal pregnancies (n = 92). Median (range) hPL mRNA was significantly higher in women with placenta previa, 782 (10-2301) copies/mL of plasma, and in those with invasive placenta, 615 (522-2102) copies/mL of plasma, when compared to normal pregnancies, 90 (4-4407) copies/mL of plasma, P < .01 and P < .05, respectively. We found a sensitivity of 100% and a specificity of 61.5% for the prediction of invasive placenta among women with placenta previa. In conclusion, expression of hPL mRNA is increased in plasma of women with placenta previa and invasive placenta at 28 to 32 weeks of gestation.