Cytoprotective nanoparticles by conjugation of a polyhis tagged annexin V to a nanoparticle drug.
Cytoprotective nanoparticles by conjugation of a polyhis tagged annexin V to a nanoparticle drug.
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通过将多聚组氨酸标记的膜联蛋白 V 与纳米颗粒药物缀合来提供细胞保护纳米颗粒。
DOI：
10.1039/c4nr06861k
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发表时间：
2015
期刊：
影响因子：
6.7
通讯作者：
Josephson,Lee
中科院分区：
文献类型：
--
作者：
Chen,HowardH;Yuan,Hushan;Cho,Hoonsung;Sosnovik,DavidE;Josephson,Lee
我们通过使马来酰亚胺官能化的Feraheme（FH）与多组氨酸标记的膜联蛋白V的二硫键连接的二聚体反应来合成细胞保护性磁性纳米颗粒。（通过激光散射，直径= 28.0 ± 2.0 nm，7.6膜联蛋白/纳米颗粒）对经受质膜破坏化疗或机械应力的细胞具有细胞保护性，并且比起始膜联蛋白V具有显著更高的保护性。膜联蛋白-纳米颗粒提供了设计纳米材料的方法，所述纳米材料拮抗坏死的质膜渗透性特征，并且可以用作细胞保护剂。
We synthesized a cytoprotective magnetic nanoparticle by reacting a maleimide functionalized Feraheme (FH) with a disulfide linked dimer of a polyhis tagged annexin V. Following reductive cleavage of disulfide, the resulting annexin-nanoparticle (diameter = 28.0 ± 2.0 nm by laser light scattering, 7.6 annexin's/nanoparticle) was cytoprotective to cells subjected to plasma membrane disrupting chemotherapeutic or mechanical stresses, and significantly more protective than the starting annexin V. Annexin-nanoparticles provide an approach to the design of nanomaterials which antagonize the plasma membrane permeability characteristic of necrosis and which may have applications as cytoprotective agents.