MicroRNA-27a promotes myoblast proliferation by targeting myostatin
MicroRNA-27a promotes myoblast proliferation by targeting myostatin
复制标题
MicroRNA-27a 通过靶向肌生长抑制素促进成肌细胞增殖
DOI：
10.1016/j.bbrc.2012.05.106
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发表时间：
2012-06-29
影响因子：
3.1
通讯作者：
Chen, Daiwen
中科院分区：
文献类型：
--
作者：
Huang, Zhiqing;Chen, Xiaoling;Chen, Daiwen
microRNA（miRNAs）是一类内源性非编码RNA，在骨骼肌发育以及调节肌细胞增殖和分化中发挥重要作用。然而，miRNAs在成肌细胞增殖中的作用仍然知之甚少。我们发现miR-27 a在C2 C12成肌细胞增殖过程中表达增加。此外，miR-27 a在C2 C12细胞中的过表达通过降低骨骼肌生成的关键抑制剂肌生成抑制素的表达来促进成肌细胞增殖。此外，通过荧光素酶报告基因分析证实miR-27 a靶向肌肉生长抑制素3 'UTR。总之，这些结果表明miR-27 a通过靶向肌生长抑制素促进成肌细胞增殖。(C)2012 Elsevier Inc. All rights reserved.
MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs that play critical roles in skeletal muscle development as well as in regulation of muscle cell proliferation and differentiation. However, the role of miRNAs in myoblast proliferation remains poorly understood. Here we found that the expression of miR-27a was increased during proliferation of C2C12 myoblasts. Moreover, overexpression of miR-27a in C2C12 cells promoted myoblast proliferation by reducing the expression of myostatin, a critical inhibitor of skeletal myogenesis. In addition, the miR-27a was confirmed to target myostatin 3'UTR by a luciferase reporter analysis. Together, these results suggest that miR-27a promotes myoblast proliferation through targeting myostatin. (C) 2012 Elsevier Inc. All rights reserved.