Expression of a novel cardiac-specific tropomyosin isoform in humans
Expression of a novel cardiac-specific tropomyosin isoform in humans
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DOI：
10.1016/j.bbrc.2004.06.084
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发表时间：
2004-08-06
影响因子：
3.1
通讯作者：
Dube, DK
中科院分区：
文献类型：
--
作者：
Denz, CR;Narshi, A;Dube, DK
原肌球蛋白是由一组高度保守的基因编码的肌动蛋白结合蛋白家族。人类有四个原肌球蛋白编码基因：TPM 1，TPM 2，TPM 3和TPM 4，已知每个基因通过选择性剪接产生多种亚型。启动子和3 ′末端加工。TPM 1是最通用的并且编码多种组织特异性同种型。横纹肌特异性的TPM 1亚型，命名为TPM 1 α，由10个外显子组成：1a、2 B、3、4、5、6 B、7、8和9a/B。在这项研究中，使用RT-PCR与成人和胎儿的人RNA，我们提出的证据表明，一种新的异构体的TPM 1基因，是专门表达在心脏组织中。新的异构体被命名为TPM 1 kappa，包含外显子2a而不是2b。人GFP. TPM 1 kappa融合蛋白的异位表达可促进缺乏原肌球蛋白的心脏突变型蝾螈心肌的肌原纤维形成。(C)2004年爱思唯尔公司All rights reserved.
Tropomyosins are a family of actin binding proteins encoded by a group of highly conserved genes. Humans have four tropomyosin-encoding genes: TPM1, TPM2, TPM3, and TPM4, each of which is known to generate multiple isoforms by alternative splicing. promoters, and 3' end processing. TPM1 is the most versatile and encodes a variety of tissue specific isoforms. The TPM1 isoform specific to striated muscle, designated TPM1alpha, consists of 10 exons: 1a, 2b, 3, 4, 5 6b, 7, 8, and 9a/b. In this study, Using RT-PCR with adult and fetal human RNAs, we present evidence for the expression of a novel isoform of the TPM1 gene that is specifically expressed in cardiac tissues. The new isoform is designated TPM1kappa and contains exon 2a instead of 2b. Ectopic expression of human GFP.TPM1kappa fusion protein can promote myofibrillogenesis in cardiac mutant axolotl hearts that are lacking in tropomyosin. (C) 2004 Elsevier Inc. All rights reserved.