Dual regulation of iron deficiency response mediated by the transcription factor IDEF1
Dual regulation of iron deficiency response mediated by the transcription factor IDEF1
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DOI：
10.4161/psb.5.2.10459
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发表时间：
2010-01-01
影响因子：
2.9
通讯作者：
Nishizawa, Naoko K.
中科院分区：
文献类型：
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作者：
Kobayashi, Takanori;Nakanishi, Hiromi;Nishizawa, Naoko K.
高等植物通过调控铁稳态相关基因的表达来响应铁供应的波动。植物对缺铁的转录反应是通过顺式作用元件和反式作用因子之间的各种相互作用来介导的。转录因子IDEF 1通过识别缺铁应答顺式作用元件IDE 1内的CATGC序列来调节水稻对缺铁的应答。我们最近提出的证据表明，IDEF 1介导的两个阶段的反应，铁缺乏。在缺铁的早期阶段，大多数已知的铁吸收/利用相关基因的正调控IDEF 1。在随后的阶段中，IDEF 1介导的这些铁吸收/利用相关基因的调节不太明显。反过来，表达的几个铁缺乏诱导的基因编码胚胎发生后期丰富的蛋白质越来越多地调节IDEF 1在随后的阶段。我们提出IDEF 1在缺铁反应中的双重功能，即（1）通过含CATGC的IDE 1-like元件协调铁利用相关基因的反式激活，特别是在缺铁的早期阶段;（2）通过RY元件反式激活种子成熟相关基因，特别是在缺铁的后续阶段。IDEF 1似乎已经进化到介导铁缺乏诱导和种子成熟相关基因表达之间的界面。
Higher plants respond to fluctuating Fe availability by regulating the expression of genes involved in Fe homeostasis. Transcriptional responses to Fe deficiency in plants are mediated via various interactions between cis-acting elements and trans-acting factors. The transcription factor IDEF1 regulates the response to Fe deficiency in Oryza sativa (rice) by recognizing the CATGC sequence within the Fe deficiency-responsive cis-acting element IDE1. We recently presented evidence that IDEF1 mediates two-phase responses to Fe deficiency. During the early stages of Fe deficiency, the majority of known Fe uptake/utilization-related genes are positively regulated by IDEF1. In subsequent stages, IDEF1-mediated regulation of these Fe uptake/utilization-related genes are less obvious. In turn, expression of several Fe deficiency-induced genes encoding late embryogenesis abundant proteins is increasingly regulated by IDEF1 at the subsequent stages. We propose a dual function of IDEF1 for Fe deficiency response, namely, (1) the coordinated transactivation of Fe utilization-related genes via CATGC-containing IDE1-like elements, especially at the early stage, and (2) the transactivation of seed maturation-related genes via RY elements, especially during the subsequent stages of Fe deficiency. IDEF1 appears to have evolved to mediate an interface between Fe deficiency-inducible and seed maturation-related gene expression.