BIODEGRADATION OF 4-CHLORO-2-METHYLPHENOXYACETIC ACID BY ENDOPHYTIC FUNGUS PHOMOPSIS SP. IN LIQUID MEDIUM AND SOIL
BIODEGRADATION OF 4-CHLORO-2-METHYLPHENOXYACETIC ACID BY ENDOPHYTIC FUNGUS PHOMOPSIS SP. IN LIQUID MEDIUM AND SOIL
复制标题
内生真菌拟茎点霉对 4-氯-2-甲基苯氧基乙酸的生物降解。
DOI：
10.15666/aeer/1902_12951307
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发表时间：
2021-01-01
影响因子：
0.7
通讯作者：
Zeng, D.
中科院分区：
文献类型：
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作者：
Wang, Y.;Feng, G.;Zeng, D.
从番石榴中分离到一株能有效降解4-氯-2-甲基苯氧基乙酸(MCPA)的内生真菌。根据其表型特征和rDNA基因序列分析，该分离株为拟南星霉属。并命名为E41。对影响该菌株降解MCPA的介质pH、温度、MCPA初始浓度等因素进行了优化。在MCPA浓度为50 mg/L、温度为30℃、pH为6的最佳条件下，接种7d的降解率最高可达86.89%。在生物修复实验中，菌株E41的存在显著促进了MCPA在所有土壤中的生物降解。这些结果表明，菌株E41在MCPA污染环境的生物修复方面具有潜在的应用价值。
An endophytic fungus strain, newly isolated from Psidium guajava (common guava), could effectively degrade 4-chloro-2-methylphenoxyacetic acid (MCPA). According to the analysis of its phenotypic feature and ITS rDNA gene sequence, the isolated strain was identified as Phomopsis sp. and named E41. The influencing factors including medium pH, temperature, and initial concentration of MCPA affecting the degradation of MCPA by this strain were optimized in this study. The maximum biodegradation rate of 86.89% was obtained after inoculation for 7 days under the optimal conditions with MCPA concentration of 50 mg/L, temperature 30 ?C and a pH of 6. The metabolite 4-chloro-2-methylphenol was detected by using gas chromatography-mass spectrometry (GC-MS). In the bioremediation experiment, the biodegradation of MCPA was significantly enhanced in all soils with the presence of the strain E41. These results suggested that the strain E41 may have potential value for the bioremediation of MCPA-contaminated environment.