Immune stimulation reduces sleep and memory ability in Drosophila melanogaster
Immune stimulation reduces sleep and memory ability in Drosophila melanogaster
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免疫刺激降低果蝇的睡眠和记忆能力
作者：
Eamonn B. Mallon;A. Alghamdi;R. Holdbrook;E. Rosato
心理神经免疫学研究神经生物学，免疫学和行为之间越来越多的联系。我们证明了免疫反应对两种基本行为的影响：黑腹果蝇的睡眠和记忆能力。我们使用Geneswitch系统上调肽聚糖受体蛋白（PGRP）的表达，从而在没有感染的情况下刺激免疫系统。Geneswitch通过向果蝇喂食类固醇RU 486来激活。我们使用了一个令人厌恶的经典条件反射范式来量化记忆和活动的措施来推断睡眠。免疫刺激的果蝇表现出睡眠水平降低，这不能用清醒活动的普遍增加来解释。免疫刺激的果蝇也表现出记忆能力的下降。这些结果支持果蝇作为免疫-神经相互作用的模型，并为睡眠在免疫反应和记忆之间的相互作用提供了可能的作用。
Psychoneuroimmunology studies the increasing number of connections between neurobiology, immunology and behaviour. We demonstrate the effects of the immune response on two fundamental behaviours: sleep and memory ability in Drosophila melanogaster. We used the Geneswitch system to upregulate peptidoglycan receptor protein (PGRP) expression, thereby stimulating the immune system in the absence of infection. Geneswitch was activated by feeding the steroid RU486, to the flies. We used an aversive classical conditioning paradigm to quantify memory and measures of activity to infer sleep. Immune stimulated flies exhibited reduced levels of sleep, which could not be explained by a generalised increase in waking activity. Immune stimulated flies also showed a reduction in memory abilities. These results lend support to Drosophila as a model for immune–neural interactions and provide a possible role for sleep in the interplay between the immune response and memory.