RESTORATION OF T-CELL DEVELOPMENT IN RAG-2 DEFICIENT MICE BY FUNCTIONAL TCR TRANSGENES
RESTORATION OF T-CELL DEVELOPMENT IN RAG-2 DEFICIENT MICE BY FUNCTIONAL TCR TRANSGENES
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DOI：
10.1126/science.8430336
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发表时间：
1993-02-05
期刊：
影响因子：
56.9
通讯作者：
ALT, FW
中科院分区：
文献类型：
--
作者：
SHINKAI, Y;KOYASU, S;ALT, FW
将TCR α转基因、TCR β转基因或两者引入RAG-2-/-小鼠中差异性地挽救T细胞发育。RAG-2-/-小鼠具有少量细胞内表达CD 3 γ δ和ζ蛋白的TCR-CD 4-CD 8-（双阴性，DN）胸腺细胞。将TCR β转基因而非TCR α转基因引入RAG-2-/-背景中恢复了胸腺细胞的正常数量。这些细胞是CD 4 + CD 8+（双阳性，DP），并表达少量与CD 3 γ δ结合的表面TCR β链二聚体，但不表达ζ。表达α和β TCR转基因的RAG-2-/-小鼠产生DP和单阳性胸腺细胞。因此，TCR β亚基，可能与一种新的CD 3复合物结合，参与DN向DP的转变。
Introduction of TCRalpha transgene, TCRbeta transgene, or both into RAG-2-/- mice differentially rescues T cell development. RAG-2-/- mice have small numbers of TCR-CD4-CD8- (double negative, DN) thymocytes that express CD3gammadeltaepsilon and zeta proteins intracellularly. Introduction of a TCRbeta transgene, but not a TCRalpha transgene, into the RAG-2-/- background restored normal numbers of thymocytes. These cells were CD4+CD8+ (double positive, DP) and expressed small amounts of surface TCRbeta chain dimers in association with CD3gammadeltaepsilon but not zeta. RAG-2-/- mice that expressed alpha and beta TCR transgenes developed both DP and single positive thymocytes. Thus, the TCRbeta subunit, possibly in association with a novel CD3 complex, participates in the DN to the DP transition.