Mixed exposure effect of seminal metals on semen quality, mediation of total antioxidant capacity, and moderation of GSTM1/GSTT1 gene deletion in Chinese reproductive-aged men.
Mixed exposure effect of seminal metals on semen quality, mediation of total antioxidant capacity, and moderation of GSTM1/GSTT1 gene deletion in Chinese reproductive-aged men.
复制标题
DOI：
10.1016/j.envres.2023.115888
复制
发表时间：
2023-04
影响因子：
8.3
通讯作者：
Jing-chao Ren;Huidong Jin;Chen Zhang;Song Liu;Jinyan Xi;jinhu Cao;fenglong Lin;Yufen Han;Pingyang Zhang;Fengquan Zhang;Guang-hui Zhang;Han Yang;Heng-Feng Huang;Jia Cao
中科院分区：
文献类型：
--
作者：
Jing-chao Ren;Huidong Jin;Chen Zhang;Song Liu;Jinyan Xi;jinhu Cao;fenglong Lin;Yufen Han;Pingyang Zhang;Fengquan Zhang;Guang-hui Zhang;Han Yang;Heng-Feng Huang;Jia Cao
背景金属暴露对精液质量的影响以及氧化损伤在这一过程中的作用尚不清楚。方法我们招募了825名中国男性志愿者，测定了12种精液金属(锰、铜、锌、硒、镍、镉、铅、钴、银、钡、铊和铁)、总抗氧化能力(TAC)和还原型谷胱甘肽(GSH)。同时检测精液参数和GSTM1/GSTT1缺失型。应用贝叶斯核机器回归(BKMR)方法评价混合接触金属对精液参数的影响。分析TAC对GSTM1/GSTT1缺失的调节作用。结果大多数精液金属浓度之间存在相关性。BKMR模型显示精液量与金属混合物呈负相关，Cd(CPIP=0.60)和Mn(CPIP=0.10)是主要贡献者。与将所有去鳞金属固定在其中位数(50%百分位数)相比，将去鳞金属固定在其第75%百分位数时，TAC值降低了2.17个单位(95%CI：−2.60，−1.75)。中介分析表明，锰降低了精液量，其中27.82%由TAC介导。BKMR模型和多元线性模型均显示精液镍与精子密度、精子总数和前向活动率呈负相关，并被GSTM1/GSTT1修正。此外，在GSTT1和GSTM1缺失的男性中，精液镍与精子总数呈负相关(β[95%CI]：0.328[-0.521，−0.136])，而在GSTT1和/或GSTM1缺失的男性中，精液镍与精子总数呈负相关。虽然Fe与精子密度和精子总数呈正相关，但单因素分析显示，Fe与精子密度和精子总数呈反“U”型关系。结论精液中12种金属的暴露水平与精液量呈负相关，Cd和Mn是主要贡献者。TAC可能参与了这一过程。GSTT1和GSTM1可以改善精液镍暴露引起的精子总数的减少。
BackgroundThe effects of metal exposure on semen quality and the role of oxidative damage in this process remain unclear.MethodsWe recruited 825 Chinese male volunteers, and 12 seminal metals (Mn, Cu, Zn, Se, Ni, Cd, Pb, Co, Ag, Ba, Tl, and Fe), the total antioxidant capacity (TAC), and reduced glutathione were measured. Semen parameters andGSTM1/GSTT1-null genotypes were also detected. Bayesian kernel machine regression (BKMR) was applied to evaluate the effect of the mixed exposure to metals on semen parameters. The mediation of TAC and moderation ofGSTM1/GSTT1deletion were analyzed.ResultsMost seminal metal concentrations were correlated with each other. The BKMR models revealed a negative association between the semen volume and metal mixture, with Cd (cPIP = 0.60) and Mn (cPIP = 0.10) as the major contributors. Compared to fixing all scaled metals at their median value (50th percentiles), fixing the scaled metals at their 75th percentiles decreased the TAC by 2.17 units (95%CI: −2.60, −1.75). Mediation analysis indicated that Mn decreased the semen volume, with 27.82% of this association mediated by TAC. Both the BKMR and multi-linear models showed that seminal Ni was negatively correlated with sperm concentration, total sperm count, and progressive motility, which was modified byGSTM1/GSTT1.Furthermore, Ni and the total sperm count showed a negative association inGSTT1andGSTM1null males (β[95%CI]: 0.328 [-0.521, −0.136]) but not in males withGSTT1and/orGSTM1. Although Fe and the sperm concentration and total sperm count were positively correlated, they showed inverse “U” shapes in univariate analysis.ConclusionExposure to the 12 metals was negatively associated with semen volume, with Cd and Mn as the major contributors. TAC may mediate this process.GSTT1andGSTM1can modify the reduction in the total sperm count caused by seminal Ni exposure.