Prevalence and Antifungal Susceptibility of Pathogenic Yeasts in China: A 10-Year Retrospective Study in a Teaching Hospital
Prevalence and Antifungal Susceptibility of Pathogenic Yeasts in China: A 10-Year Retrospective Study in a Teaching Hospital
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DOI：
10.3389/fmicb.2020.01401
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发表时间：
2020-07-03
影响因子：
5.2
通讯作者：
Li, Ruoyu
中科院分区：
文献类型：
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作者：
Song, Yinggai;Chen, Xianlian;Li, Ruoyu
为了确定中国三级医院病原菌流行率和抗真菌药敏模式的动态变化，我们分析了2010年1月至2019年12月北京大学医学真菌学研究中心保存的527株酵母菌，并通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）和核糖体DNA测序正确鉴定了19种酵母菌。使用含有9种临床可用抗真菌药物的Sensititre YeastOne比色微稀释板进行抗真菌药敏试验。使用临床和实验室标准协会（CLSI）批准的标准M27-A3 （S4）和新修订的临床断点或物种特异性和方法特异性流行病学截止值来解释药敏试验数据。在本研究中，虽然白色念珠菌是优势的单一种，但非c。白念珠菌种在10年中有6年占分离物的50%以上，近5年出现了更多的稀有种。非。最常见的白色念珠菌属有严格感假丝酵母菌、热带假丝酵母菌和光滑假丝酵母菌。c区氟康唑和伏立康唑耐药情况。敏感型紧致残蝇种群
To determine the dynamic changes of pathogenic yeast prevalence and antifungal susceptibility patterns in tertiary hospitals in China, we analyzed 527 yeast isolates preserved in the Research Center for Medical Mycology at Peking University, Beijing, China, between Jan 2010 and Dec 2019 and correctly identified 19 yeast species by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and ribosomal DNA sequencing. Antifungal susceptibility testing was performed following a Sensititre YeastOne colorimetric microdilution panel with nine clinically available antifungals. The Clinical and Laboratory Standards Institute (CLSI)-approved standard M27-A3 (S4) and newly revised clinical breakpoints or species-specific and method-specific epidemiological cutoff values were used for the interpretation of susceptibility test data. In this study, althoughCandida albicanswas the predominant single species, non-C. albicansspecies constituted >50% of isolates in 6 out of 10 years, and more rare species were present in the recent 5 years. The non-C. albicansspecies identified most frequently wereCandida parapsilosis sensu stricto,Candida tropicalis, andCandida glabrata. The prevalence of fluconazole and voriconazole resistance in theC. parapsilosis sensu strictopopulation was