CORRELATION OF PATTERNS OF ANCHORAGE-INDEPENDENT GROWTH WITH INVIVO BEHAVIOR OF CELLS FROM A MURINE FIBRO-SARCOMA
CORRELATION OF PATTERNS OF ANCHORAGE-INDEPENDENT GROWTH WITH INVIVO BEHAVIOR OF CELLS FROM A MURINE FIBRO-SARCOMA
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DOI：
10.1073/pnas.77.2.1039
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
FIDLER, IJ
中科院分区：
文献类型：
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作者：
CIFONE, MA;FIDLER, IJ
小鼠UV-2237纤维肉瘤肿瘤细胞的体外锚定非依赖性生长模式与其在体内产生实验性转移的能力相关。当接种到0.3%高贵琼脂半固体培养基中时，转移性克隆的细胞以比低转移潜能克隆的细胞更快的速度发育成更大的肿瘤集落。当将肿瘤细胞接种到0.6%高贵琼脂中时，低转移潜能克隆细胞的集落发育几乎完全受到限制。将来自异质亲本UV-2237纤维肉瘤的肿瘤细胞接种到含有0.6%琼脂半固体培养基的培养皿中。在单独的实验中，2周后分离出16个集落，并在单层培养物中建立为单个细胞系。通过体内肺集落测定法测定，所有这些细胞系均产生实验性转移。这些数据表明，锚定非依赖性生长的UV-2237肿瘤细胞在0.6%高贵的琼脂半固体培养基是有选择性的，并允许转移性亚群的隔离。
The pattern of in vitro anchorage-independent growth of tumor cells from the murine UV-2237 fibrosarcoma correlated with their ability to produce experimental metastasis in vivo. When seeded into 0.3% noble agar semisolid medium, cells of metastatic clones developed into larger tumor colonies at a faster rate than did cells of clones with low metastatic potential. When tumor cells were plated into 0.6% noble agar, colony development by cells of low metastatic potential clones was almost completely restricted. Tumor cells from the heterogeneous parent UV-2237 fibrosarcoma were plated into dishes containing 0.6% agar semisolid medium. In separate experiments, 16 colonies were isolated 2 wk thereafter and were established as individual cell lines in monolayer cultures. All of these cell lines produced experimental metastases as determined by in vivo lung colony assay. The data suggest that anchorage-independent growth of UV-2237 tumor cells in 0.6% noble agar semisolid medium is selective and permits the isolation of metastatic subpopulations.