Modulation by recombinant DNA leukocyte (alpha) and fibroblast (beta) interferons of the expression and shedding of HLA- and tumor-associated antigens by human melanoma cells.
Modulation by recombinant DNA leukocyte (alpha) and fibroblast (beta) interferons of the expression and shedding of HLA- and tumor-associated antigens by human melanoma cells.
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DOI：
10.4049/jimmunol.133.3.1649
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发表时间：
1984-09
影响因子：
4.4
通讯作者：
P. Giacomini;A. Aguzzi;S. Pestka;P. Fisher;S. Ferrone
中科院分区：
文献类型：
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作者：
P. Giacomini;A. Aguzzi;S. Pestka;P. Fisher;S. Ferrone
用一组单克隆抗体，重组人白细胞干扰素（即，IFN-α A，IFN-α D），杂合白细胞IFN（即，IFN-α A/D（Bg 1））和重组成纤维细胞（β）IFN对培养的黑素瘤细胞系科洛38的四种类型的黑素瘤相关抗原（MAA）和HLA抗原的表达和脱落的影响。IFN均不影响高分子量的表达。黑色素瘤相关抗原（HMW-MAA），但它们都增加了其脱落。干扰素增加了115，000个MAA和100，000个MAA的表达和脱落;不同干扰素制剂的作用大小和动力学不同。细胞质MAA是最敏感的IFN调制，因为所有四种类型的增加其表面表达，其总含量，其脱落。三种白细胞干扰素和成纤维细胞干扰素均能增加HLA-A、B、C抗原的表达，对游离重链的作用比对HLA-A、B、C复合物的作用更明显。但只有白细胞IFN能促进HLA-A、B、C分子复合物的脱落。三种类型的白细胞IFN，特别是成纤维细胞IFN增强HLA-D区的基因产物的表达，效果更显着的DC-1抗原比HLA-DR抗原。未检测到对HLA-DR和DC-1抗原脱落的影响。
With a panel of monoclonal antibodies, the effect of recombinant human leukocyte interferons (i.e., IFN-alpha A, IFN-alpha D), of a hybrid leukocyte IFN (i.e., IFN-alpha A/D (Bg1)), and of recombinant fibroblast (beta) IFN on the expression and shedding of four types of melanoma-associated antigens (MAA) and of HLA antigens by the cultured melanoma cell line Colo 38 was investigated. None of the IFN affected the expression of the high m.w. melanoma-associated antigen (HMW-MAA), but all of them increased its shedding. The expression and shedding of the 115,000 MAA and of the 100,000 MAA were increased by IFN; the magnitude of the effect as well as the kinetics were different for the various IFN preparations. The cytoplasmic MAA was the most sensitive to modulation by IFN, because all four types increased its surface expression, its total content, and its shedding. The three types of leukocyte IFN, as well as the fibroblast IFN, were all effective in increasing the expression of HLA-A,B,C antigens, the effect being more marked on the free heavy chain than on the HLA-A,B,C complex. However, only leukocyte IFN enhanced the shedding of the HLA-A,B,C molecular complex. The three types of leukocyte IFN and especially fibroblast IFN enhanced the expression of the gene products of the HLA-D region, the effect being more marked on DC-1 antigens than on HLA-DR antigens. No effect on the shedding of HLA-DR and DC-1 antigens was detected.