Phosphite cannot be used as a phosphorus source but is non-toxic for microalgae
Phosphite cannot be used as a phosphorus source but is non-toxic for microalgae
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DOI：
10.1016/j.plantsci.2014.11.015
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发表时间：
2015-02-01
期刊：
影响因子：
5.2
通讯作者：
Herrera-Estrella, Luis
中科院分区：
文献类型：
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作者：
Loera-Quezada, Maribel M.;Leyva-Gonzalez, Marco Antonio;Herrera-Estrella, Luis
磷（P）作为RNA、DNA和磷脂的结构组分对所有生物体起着关键作用。微藻是一种自养生物，据报道，它们只能吸收完全氧化的磷酸盐（Pi）作为磷源。然而，也有微生物能够利用P还原化合物（即亚磷酸盐（Phi）和次磷酸盐）作为唯一的P源，如细菌和蓝藻，在这项研究中，我们评估是否微藻，如衣藻reinhardtii，Botryocaccus braunii和Ettlia olioabundans，能够使用Phi作为唯一的P源。我们的研究表明，这三种微藻不能使用Phi作为唯一的P源。我们还发现，当Phi的浓度等于或高于Pi时，Phi对C有抑制作用。莱氏生长然而，自C. reinhardtii能够在高浓度Phi存在下存活长时间培养，并在转移到含Pi的培养基后恢复细胞分裂能力，我们注意到Phi对这种微生物没有毒性。我们认为Phi对C. reinhardtii增长可能是造成的，至少部分，由运输的Pi和Phi之间的竞争。(C)2014作者由Elsevier爱尔兰有限公司发布。这是CC BY-NC-SA许可证下的开放获取文章（http：//creativecommons.org/licenses/by-nc-sa/3.0/）。
Phosphorous (P) plays a critical role for all living organisms as a structural component of RNA, DNA and phospholipids. Microalgae are autotrophs organisms that have been reported to only assimilate the fully oxidized phosphate (Pi) as P source. However, there are microorganisms capable of utilizing P reduced compounds (i.e. phosphite (Phi) and hypophosphite) as a sole P source, such as bacteria and cyanobacteria,In this study, we evaluated whether microalgae, such as Chlamydomonas reinhardtii, Botryocaccus braunii and Ettlia oleoabundans, are capable of using Phi as a sole P source. Our studies revealed that these three microalgae are unable to use Phi as a sole P source. We also found that when Phi is present at concentrations equal or higher than that of Pi, Phi has an inhibitory effect on C. reinhardtii growth. However, since C. reinhardtii was able to survive for a long period of cultivation in the presence of high concentrations of Phi and to recover cell division capacity after transfer to media containing Pi, we noticed that Phi is not toxic for this microalga. We propose that the inhibitory effect of Phi on C. reinhardtii growth might be caused, at least in part, by a competition between the transport of Pi and Phi. (C) 2014 The Authors. Published by Elsevier Ireland Ltd. This is an open access article under the CC BY-NC-SA license (http://creativecommons.org/licenses/by-nc-sa/3.0/).