Detection of dengue virus in saliva and urine by real time RT-PCR.
Detection of dengue virus in saliva and urine by real time RT-PCR.
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DOI：
10.1186/1743-422x-7-22
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发表时间：
2010-01-27
期刊：
影响因子：
4.8
通讯作者：
Aquino VH
中科院分区：
文献类型：
--
作者：
Poloni TR;Oliveira AS;Alfonso HL;Galvão LR;Amarilla AA;Poloni DF;Figueiredo LT;Aquino VH
登革热病毒（DENV）感染的早期诊断对于患者管理和控制登革热疫情非常重要。本研究的目的是分析尿液和唾液样本通过实时 RT-PCR 早期诊断 DENV 感染的有用性。该研究包括两名曾在圣保罗大学里贝朗普雷图医学院总医院就诊的发热患者。对两名患者采集的血清、尿液和唾液样本进行实时 RT-PCR 检测和量化 DENV。在两名患者的血清、尿液和唾液样本中均检测到登革热 RNA。患者 1 感染了 DENV-2，患者 2 感染了 DENV-3。本研究中提供的数据表明，当难以获得血液样本时（例如新生儿和出血综合征患者），尿液和唾液可以用作早期诊断登革热病毒感染的替代样本。
Early diagnosis of dengue virus (DENV) infection is important for patient management and control of dengue outbreaks. The objective of this study was to analyze the usefulness of urine and saliva samples for early diagnosis of DENV infection by real time RT-PCR. Two febrile patients, who have been attended at the General Hospital of the School of Medicine of Ribeirao Preto, Sao Paulo University were included in the study. Serum, urine and saliva samples collected from both patients were subjected to real time RT-PCR for DENV detection and quantification. Dengue RNA was detected in serum, urine and saliva samples of both patients. Patient 1 was infected with DENV-2 and patient 2 with DENV-3. Data presented in this study suggest that urine and saliva could be used as alternative samples for early diagnosis of dengue virus infection when blood samples are difficult to obtain, e.g., in newborns and patients with hemorrhagic syndromes.