NADH oxidation using modified electrodes based on lactate and glucose dehydrogenase entrapped between an electrocatalyst film and redox catalyst-modified polymers
NADH oxidation using modified electrodes based on lactate and glucose dehydrogenase entrapped between an electrocatalyst film and redox catalyst-modified polymers
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DOI：
10.1007/s00604-012-0797-2
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发表时间：
2012-06-01
影响因子：
5.7
通讯作者：
Schuhmann, Wolfgang
中科院分区：
文献类型：
--
作者：
Al-Jawadi, Eman;Poeller, Sascha;Schuhmann, Wolfgang
研究了聚亚甲基蓝（pMB）或聚亚甲基绿色（pMG）电聚合层与专门设计的甲苯胺蓝或耐尔蓝改性的甲基丙烯酸酯基电沉积聚合物组合的电极结构上的电催化NADH氧化。NAD（+）依赖性乳酸脱氢酶或NAD（+）依赖性葡萄糖脱氢酶被截留在pMB或pMG的初级电聚合层与基于甲基丙烯酸酯的氧化还原聚合物之间。评价聚合物主链和聚合物结合的氧化还原染料的组成，并且可以证明，电聚合的pMB或pMG层与染料改性的基于甲基丙烯酸酯的氧化还原聚合物的组合与单独的任一组分相比显示出上级的性质。NADH在0 mV（相对于Ag/AgCl/KCl 3 M）的外加电位下被氧化，在底物饱和时，基于乳酸脱氢酶和葡萄糖脱氢酶的修饰电极分别获得17 μ A中心点cm（-2）和28 μ A中心点cm（-2）的电流密度。
Electrocatalytic NADH oxidation was investigated at an electrode architecture involving an electropolymerized layer of poly(methylene blue) (pMB) or poly(methylene green) (pMG) in combination with specifically designed toluidine blue or nile blue modified methacrylate-based electrodeposition polymers. Either NAD(+)-dependent lactate dehydrogenase or NAD(+)-dependent glucose dehydrogenase were entrapped between the primary electropolymerized layer of pMB or pMG and the methacrylate-based redox polymer. The composition of the polymer backbone and the polymer-bound redox dye was evaluated and it could be demonstrated that the combination of the electropolymerized pMB or pMG layer together with the dye modified methacrylate-based redox polymer shows superior properties as compared with either of the components alone. NADH was oxidized at an applied potential of 0 mV vs Ag/AgCl/KCl 3 M and current densities of 17 mu A center dot cm(-2) and 28 mu A center dot cm(-2) were obtained for modified electrodes based on lactate dehydrogenase and glucose dehydrogenase, respectively, at substrate saturation.