Changes in growth factor levels in human wound fluid.
Changes in growth factor levels in human wound fluid.
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发表时间：
1992-07
期刊：
影响因子：
3.8
通讯作者：
V. Dvonch;R. Murphey;J. Matsuoka;Gary R. Grotendorst
中科院分区：
文献类型：
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作者：
V. Dvonch;R. Murphey;J. Matsuoka;Gary R. Grotendorst
研究背景：血小板源性生长因子（PDGF）在创伤愈合中起着重要作用。对人类伤口液的分析显示，术后立即存在血小板源性生长因子AA（30 kd）和单核细胞/巨噬细胞衍生生长因子（MDGF）（12至14 kd）。方法采用Westernblot法检测PDGF-AA的表达量。在反应细胞系上测定含有PDGF AA和MDGF的纯化伤口液的趋化和促有丝分裂潜力。MDGF的生物活性用对伤口液中发现的PDGF AA无反应的细胞系测定。结果术后即刻血药浓度和生物活性最高，术后24 h降至可忽略水平。MDGF的趋化活性在术后立即达到最高，并在最初24小时内以类似于PDGF AA和MDGF活性组合的方式下降。结论：这些数据表明，随着时间的推移，人伤口液中的PDGF和MDGF水平的变化，支持伤口修复的级联模型。通过证明MDGF作用于对伤口液中发现的PDGF AA无反应的细胞系，这些数据表明MDGF也可能在伤口愈合中起重要作用。
BACKGROUND It has been suggested that platelet-derived growth factor (PDGF) plays a central role in wound healing. Analysis of human wound fluid revealed the presence of PDGF AA (30 kd) and monocyte/macrophage-derived growth factor (MDGF) (12 to 14 kd) in the immediate postoperative period. METHODS The amount of PDGF AA present was assayed by Western blot analysis. The chemotactic and mitogenic potential of purified wound fluid containing PDGF AA and MDGF was determined on a responsive cell line. The biologic activity of MDGF was assayed with a cell line that is unresponsive to the PDGF AA found in wound fluid. RESULTS Both the concentration and the biologic activity were highest in the immediate postoperative period and declined to negligible levels by 24 hours after surgery. The chemotactic activity of MDGF was highest in the immediate postoperative period and declined during the first 24 hours in a manner similar to that of the combined PDGF AA and MDGF activity. CONCLUSIONS These data demonstrate the changing levels of PDGF and MDGF in human wound fluid over time, supporting the cascade model of wound repair. By demonstrating that MDGF acts on cell lines unresponsive to the PDGF AA found in wound fluid, these data suggest that MDGF may also play an important role in wound healing.