Species identification of oral viridans streptococci by restriction fragment polymorphism analysis of rRNA genes.
Species identification of oral viridans streptococci by restriction fragment polymorphism analysis of rRNA genes.
复制标题
通过 rRNA 基因的限制性片段多态性分析鉴定口腔草绿色链球菌的种类。
DOI：
10.1128/jcm.31.9.2467-2473.1993
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发表时间：
1993
影响因子：
9.4
通讯作者：
Larson,CJ
中科院分区：
文献类型：
--
作者：
Rudney,JD;Larson,CJ
口腔链球菌以前被归类为血链球菌，现已分为六个遗传群。需要通过基因型鉴定这些物种的方法。本研究比较了7个S. gordonii，三个S。sanguis、4个S. oralis、3个S. mitis，一个S. crista和7个S.在以前的DNA杂交研究中分类的副anguis菌株，以及一个临床分离株。用HindIII、PvuII、HindIII和PvuII组合、EcoRI、BamHI、Aat II、AlwNI和DraII消化DNA。DNA片段与地高辛标记的cDNA探针杂交，所述探针通过逆转录大肠杆菌16 S和23 S rRNA获得。S. oralis，S. mitis和S.在Aat Ⅱ核糖体型中，所有的副鳗都显示出一条2,290 bp的分离条带，而在鳗中则没有。gordonii，S. sanguis和S.嵴。最后三组显示种特异性条带与Aat II，也与Pvu II。S. oralis与S. mitis和S. AlwNI和DraII核糖体型中的副血吸虫。S. mitis和S. parasanguis无法区分，因为它们在PvuII、AlwNI和EcoRI模式中共享多个条带。该组中的临床分离株与S非常相似。使用的所有酶。我们的研究结果表明，核糖分型可能是有用的口腔草绿色链球菌的基因型鉴定。临床分离株的初步鉴定可用AatII，然后用PvuII或AlwNI。然后可以通过将核糖体型模式与参考菌株的核糖体型模式进行比较来鉴定分离株。这种方法可以促进这些新定义物种的临床研究。
Oral streptococci formerly classified as Streptococcus sanguis have been divided into six genetic groups. Methods to identify those species by genotype are needed. This study compared restriction fragment polymorphisms of rRNA genes (ribotypes) for seven S. gordonii, three S. sanguis, four S. oralis, three S. mitis, one S. crista, and seven S. parasanguis strains classified in previous DNA hybridization studies, as well as one clinical isolate. DNA was digested with HindIII, PvuII, HindIII and PvuII combined, EcoRI, BamHI, AatII, AlwNI, and DraII. DNA fragments were hybridized with a digoxigenin-labeled cDNA probe obtained by reverse transcription of Escherichia coli 16S and 23S rRNA. S. oralis, S. mitis, and S. parasanguis all showed an isolated 2,290-bp band in AatII ribotypes that was absent from S. gordonii, S. sanguis, and S. crista. The last three groups showed species-specific bands with AatII and also with PvuII. S. oralis could be distinguished from S. mitis and S. parasanguis in AlwNI and DraII ribotypes. S. mitis and S. parasanguis could not be distinguished, since they shared multiple bands in PvuII, AlwNI, and EcoRI patterns. The clinical isolate in the panel was very similar to S. sanguis by all enzymes used. Our findings suggest that ribotyping may be useful for genotypic identification of oral viridans streptococci. Initial digests of clinical isolates might be made with AatII, followed by PvuII or AlwNI. Isolates then could be identified by comparing ribotype patterns with those of reference strains. This approach could facilitate clinical studies of these newly defined species.