Unexplained persistent lymphadenopathy in homosexual men and the acquired immune deficiency syndrome.
Unexplained persistent lymphadenopathy in homosexual men and the acquired immune deficiency syndrome.
复制标题
男同性恋者不明原因的持续性淋巴结肿大和获得性免疫缺陷综合征。
DOI：
10.1097/00005792-198505000-00005
复制
发表时间：
1985
期刊：
影响因子：
1.6
通讯作者：
Sarngadharan,MG
中科院分区：
文献类型：
--
作者：
Gold,JW;Weikel,CS;Godbold,J;Garcia,C;Urmacher,C;Cunningham-Rundles,S;Koziner,B;Pollack,M;Gallo,RC;Sarngadharan,MG
材料和方法纪念斯隆-凯特琳癌症中心的传染病服务中心对患有淋巴结肿大的患者进行了评估。淋巴结肿大的标准是两个不连续的淋巴结链中直径大于 1 厘米的淋巴结，不包括腹股沟和股骨淋巴结，由我们中的一个人记录至少 3 个月。与淋巴结病、性传播疾病史、性行为和吸毒有关的病史详细信息是通过患者填写的问卷获得的，并由我们其中一名患者与他一起审查。采用标准方法进行常规实验室测试和血清学检查。使用人胚肺（WI38）、人胚肾（HEK）、猴肾（MK）和兔肾（RK）细胞培养物对尿液、精液、唾液和血沉棕黄层进行病毒培养。当有临床指征时，进行粪便培养和虫卵和寄生虫（包括隐孢子虫）检查。通过酶联免疫吸附测定 (ELISA) 对 84 名患者的库存血清标本中的抗 HTIV-III 抗体进行了检测。光密度至少是阴性对照血清三倍的血清被认为是阳性的。阴性血清和那些给出不一致结果的血清通过蛋白质印迹技术进行测试。对每位患者最早的可用样本进行了测试，并对连续样本进行了测试
Materials and MethodsPatients with lymphadenopathy were evaluated by the Infectious Disease Service at the Memorial Sloan-Kettering Cancer Center. The criteria for lymphadenopathy were lymph nodes larger than l cm in diameter in two non-contiguous lymph node chains, excluding the inguinal and femoral nodes, documented by one of us for at least 3 months. Details of medical history pertaining to lymphadenopathy, history of sexually transmitted diseases, sexual practices, and drug use were obtained using a questionnaire filled out by the patient and reviewed with him by one of us.Routine laboratory tests and serologies were performed with standard methods. Viral cultures of urine, semen, saliva and buffy coat were performed using human embryonic lung (WI38), human embryonic kidney (HEK), monkey kidney (MK), and rabbit kidney (RK) cell cultures. Stool cultures and examinations for ova and parasites including Cryptosporidia sp were obtained when clinically indicated. Anti-HTIV–III antibody was measured in banked serum specimens of 84 patients by enzyme-linked immunosorbent assay (ELISA). Sera that gave an optical density at least three times the negative control sera were considered positive. Nega-tive sera and those giving discrepant results were tested by the Western blot technique. The earliest available specimen from each patient was tested and serial specimens were tested from