Non-specific adherence of oxide particles as a means of quantifying protein adsorption on surfaces.
Non-specific adherence of oxide particles as a means of quantifying protein adsorption on surfaces.
复制标题
氧化物颗粒的非特异性粘附作为量化表面蛋白质吸附的一种手段。
DOI：
10.1163/156856295x00599
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Leonard,EF
中科院分区：
文献类型：
--
作者：
Leduc,C;TenHove,P;Park,S;Vroman,L;Brash,J;Leonard,EF
本文报道了定量的方法，用于测量吸附到表面的蛋白质的量，该方法是特别有用的，揭示宏观空间模式的吸附。实验测试了吸附到吸附的蛋白质上的氧化铁悬浮液的有效性，以在单独的试验中指示载玻片上存在的人血浆纤维蛋白原或人血清白蛋白（HSA）的量。相应的试验，使用放射性标记的蛋白质，进行校准的量的白蛋白或纤维蛋白原吸附到类似的幻灯片出的解决方案，不同的散装浓度。使用扫描仪和图像分析程序对氧化物沉积物进行定量。从所收集的数据产生的等温线表明粘附的氧化物和蛋白质的表面浓度吸收的光之间的连续的，单调的相关性。当表面浓度以重量单位表示时，相同的相关性适用于白蛋白和纤维蛋白原。这些结果证实了氧化物沉积的模式对应于蛋白质沉积的模式，并清楚地显示了如何定性观察，如以前报道的，可以定量扫描和数字图像分析。
This paper reports quantification of a method for measuring amounts of protein adsorbed to a surface; the method is especially useful for revealing macroscopic spatial patterns of adsorption. The experiments tested the effectiveness of iron oxide suspensions adsorbed onto the adsorbed protein to indicate, in separate trials, the amount of either human plasma fibrinogen or human serum albumin (HSA) present on glass slides. Corresponding trials, using radioactively labeled proteins, were performed to calibrate the amount of either albumin or fibrinogen adsorbed onto similar slides out of solutions of varying bulk concentrations. The oxide deposits were quantified using a scanner and an image analysis program. The isotherms produced from the collected data indicate a continuous, monotonic correlation between light absorbed by adherent oxide and surface concentration of protein. The same correlation applies to albumin and fibrinogen when surface concentrations are expressed in weight units. These results confirm that patterns of oxide deposition correspond to patterns of protein deposition and show clearly how qualitative observations, such as those previously reported, can be made quantitative with scanning and digital image analysis.