Emergence of mobile tigecycline resistance gene tet(X4)-harbouring Shewanella xiamenensis in a water environment
Emergence of mobile tigecycline resistance gene tet(X4)-harbouring Shewanella xiamenensis in a water environment
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DOI：
10.1016/j.jgar.2021.12.022
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发表时间：
2022-03-01
影响因子：
4.6
通讯作者：
Suzuki, Masato
中科院分区：
文献类型：
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作者：
Trung Duc Dao;Kasuga, Ikuro;Suzuki, Masato
目的：革兰氏阴性菌中由移动的替加环素失活酶基因泰特（X）介导的替加环素耐药性是全球公共卫生的一个新问题。然而，关于泰特（X）在自然环境中的分布的信息有限。在这项研究中，我们调查了环境革兰氏阴性细菌中泰特（X）的存在。方法：2021年3月，从越南河内的城市排水系统中获得了一株碳青霉烯和替加替林耐药的厦门希瓦氏菌分离株（NESHM-VS 1）。通过长读段和短读段测序进行全基因组测序分析，得到完整的基因组序列。结果：厦门希瓦氏菌分离株NH 4 M-VS 1染色体上携带泰特（X4）基因和bla（OXA-48）碳青霉烯酶基因。泰特（X4）的侧翼是IS 91家族转座酶基因，表明泰特（X4）的获得是由这个移动的基因元件（MGE）介导的，而在bla（OXA-48）周围没有发现MGE，这与以前的发现一致，即bla（OXA-48）是由一个可移动的基因元件（MGE）介导的。（OXA-48-like）β-内酰胺酶基因是希瓦氏菌属种特异性内源性ARG。据我们所知，这是第一次报告的泰特（X4）-窝藏希瓦氏菌。分离。我们的研究结果提供了遗传证据的复杂性，临床上重要的ARGs在水环境中的细菌之间的动态。(C)2022作者由Elsevier Ltd代表国际抗微生物化疗学会发表。
Objectives: Tigecycline resistance mediated by the mobile tigecycline-inactivating enzyme gene tet(X) in Gram-negative bacteria is an emerging concern for global public health. However, limited information is available on the distribution of tet(X) in the natural environment. In this study, we investigated the presence of tet(X) in environmental Gram-negative bacteria.Methods: A carbapenem- and tigecycline-resistant Shewanella xiamenensis isolate (NUITM-VS1) was obtained from an urban drainage in Hanoi, Vietnam, in March 2021. Whole-genome sequencing analysis was performed by long- and short-read sequencing, resulting in a complete genome sequence. Antimicrobial resistance genes (ARGs) in the genome were detected based on the custom ARG database, including all known tigecycline resistance genes.Results: Shewanella xiamenensis isolate NUITM-VS1 harboured the tet(X4) gene and the bla(OXA-48) carbapenemase gene on the chromosome. tet(X4) was flanked by IS91 family transposase genes, suggesting that the acquisition of tet(X4) was mediated by this mobile gene element (MGE), whereas no MGE was found surrounding bla(OXA-48), consistent with previous findings that bla(OXA-48-like) beta-lactamase genes are species-specific intrinsic ARGs in Shewanella spp.Conclusion: To the best of our knowledge, this is the first report of a tet(X4)-harbouring Shewanella sp. isolate. Our results provide genetic evidence of the complexity of the dynamics of clinically important ARGs among bacteria in the water environment. (C) 2022 The Authors. Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.