Liposome-Boosted Peroxidase-Mimicking Nanozymes Breaking the pH Limit
Liposome-Boosted Peroxidase-Mimicking Nanozymes Breaking the pH Limit
复制标题
DOI：
10.1002/chem.202004133
复制
发表时间：
2020-11-16
影响因子：
4.3
通讯作者：
Liu, Juewen
中科院分区：
文献类型：
--
作者：
Chen, Qiaoshu;Liu, Yibo;Liu, Juewen
过氧化物酶模拟纳米酶，如Fe 3 O 4纳米颗粒是有前途的天然酶，如辣根过氧化物酶的替代品。然而，大多数这样的纳米酶仅在酸性条件下有效地工作。研究了不同脂质体对纳米酶活性的影响。通过引入带负电荷的脂质体，过氧化物酶模拟纳米酶实现了在中性甚至碱性条件下氧化3，3 '，5，5'-四甲基联苯胺（TMB），尽管对阴离子2，2 '-连氮基-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）的活性受到抑制。Fe 3 O 4纳米粒子吸附在脂质体上，而不破坏膜的完整性，证实了荧光猝灭，染料泄漏试验，和冷冻电子显微镜。通过静电相互作用稳定TMB的蓝色氧化产物被认为是活性增强的原因。这项工作将脂质引入纳米酶研究，并且它对于在中性pH下使用纳米酶也具有实际重要的应用，例如过氧化氢和葡萄糖的检测。
Peroxidase-mimicking nanozymes such as Fe3O4 nanoparticles are promising substitutes for natural enzymes like horseradish peroxidase. However, most such nanozymes work efficiently only in acidic conditions. In this work, the influence of various liposomes on nanozyme activity was studied. By introducing negatively charged liposomes, peroxidase-mimicking nanozymes achieved oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in neutral and even alkaline conditions, although the activity towards anionic 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) was inhibited. The Fe3O4 nanoparticles adsorbed on the liposomes without disrupting membrane integrity as confirmed by fluorescence quenching, dye leakage assays, and cryo-electron microscopy. Stabilization of the blue-colored oxidized products of TMB by electrostatic interactions was believed to be the reason for the enhanced activity. This work has introduced lipids to nanozyme research, and it also has practically important applications for using nanozymes at neutral pH, such as the detection of hydrogen peroxide and glucose.